利用选择导入系分析大豆芽期和苗期耐旱性的遗传重叠  

Genetic Overlap of Drought-Tolerance Loci between Germination Stage and Seedling Stage Analyzed Using Introgression Lines in Soybean

作　　者：邱鹏程[1,2] 张闻博[1,2] 李灿东[3] 蒋洪蔚[2] 刘春燕[2] 范冬梅[1] 曾庆力[1] 胡国华[2,4] 陈庆山[1] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]黑龙江省农垦科研育种中心,黑龙江哈尔滨150090 [3]黑龙江省农业科学院佳木斯分院,黑龙江佳木斯154007 [4]国家大豆工程技术研究中心,黑龙江哈尔滨150050

出　　处：《作物学报》2011年第3期477-483,共7页Acta Agronomica Sinica

基　　金：农业部转基因生物新品种培育重大专项(2009ZX08009-013B);引进国际先进农业科学技术计划(948计划)项目[2006-G1(A)]资助

摘　　要：以黑龙江主栽品种红丰11为母本,与美国品种Clark杂交,再以红丰11为轮回亲本,对回交后代的芽期和苗期的耐旱性进行筛选。结果获得芽期耐旱导入系44个,采用单项方差分析检测到10个控制芽期耐旱性的QTL;获得苗期耐旱导入系46个,检测到影响苗期叶片相对含水量、叶片持水能力、胁迫期间株高变化量的21个QTL。大多数位点的遗传是相互独立的,只有分布于A1、K、I和H连锁群上的Satt449、Satt499、Satt440和Sat_180位点是在芽期、苗期干旱条件下共同检测到的,表明芽期和苗期的耐旱性存在部分的遗传重叠。以上结果为深入研究大豆耐旱性以及进行分子设计育种以累加芽期苗期重要耐旱QTL奠定了基础。Soybean grows worldwide in many regions under drought stress,so drought tolerance（DT）is a very important trait for the crop.Most researches have focused on the QTL mapping related with drought-tolerance,but there is no research on the genetic overlap in soybean.A primary backcross introgression lines（ILs）were constructed with Hongfeng 11 as recurrent parent and Clark as donor parent.Forty-four individuals from BC1F5 introgression populations were screened out under drought stress comparing with the Hongfeng11 in germination stage,and 46 individuals were obtained under drought stress comparing with the control population in seedling stage.The QTL identification at germination stage and seedling stage was conducted by one-way ANOVA（for single marker analysis,P〈0.05）with the two introgression populations.Then 10 QTLs were mapped in germination stage,21 QTLs were mapped in seedling stage laced on relative water content（RWC）,water holding capacity（WHC）,and growth of plant height（GPH）.Four QTLs including Satt449,Satt499,Satt440,and Sat_180 in A1,K,I,and H linkage groups were mapped both in germination stage and seedling stage,indicating a partial genetic overlap between these two stages in soybean.The above results would provide a basic for fine mapping and molecular breeding for favorable genes related to DT in soybean.

关 键 词：大豆 导入系 耐旱性 遗传重叠 

分 类 号：S565.1[农业科学—作物学]