支原体鉴定药敏一体化试剂盒假阳性结果分析  

Analysis of false-positive results of mycoplasma ICS Kit.

作　　者：吴丽婷[1] 陈立刚[1] 

机构地区：[1]海南省人民医院医学检验部,海南海口570311

出　　处：《海南医学》2011年第6期110-112,共3页Hainan Medical Journal

摘　　要：目的对造成支原体液体培养法假阳性的原因进行分析。方法采用解脲支原体(Uu)-人型支原体(Mh)鉴定与药敏一体化试剂对322例妇科门诊患者分泌物进行检验,并对支原体阳性培养液进行细菌培养及鉴定,用已知可分解或不分解尿素和精氨酸的标准菌株配制成不同浓度的菌液进行干扰试验。结果 213例支原体阳性培养液中共分离出细菌14例,其中192例清的变红培养液细菌分离率为0%;21例混浊或微浊培养液培养出细菌真菌14例,分离率为66.7%。在干扰试验中,当菌液浓度<104/ml时,培养液对细菌抑制明显;当菌液浓度≥104/ml时,培养液对细菌抑制效果不理想,培养液呈不同程度混浊,如果尿素和精氨酸分解试验至少有一项阳性的细菌,可导致假阳性。结论标本中如含有大量分解尿素或精氨酸的细菌,且支原体培养液不能抑制细菌的生长时,可造成支原体培养假阳性。所以培养液颜色变红和混浊时不能直接报告支原体阳性,应结合其他检测方法与临床症状做出判断。Objective To analyze the reasons of false-positive results in broth method of mycoplasma.Methods Integration kit of Uu-Mh identification and drug susceptibility was used to test the secretion of 322 patients in gynecology clinic;furthermore,bacterial culture and identify was done in those mycoplasma positive solutions.Interference test was performed in different-density bacterial solutions prepared by standard strain known to whether decompose urea or arginine.Results In the 213 cases of mycoplasma positive solution,bacterium was separated in 14 cases,and the separation rate of bacterium was 0% in 192 cases of supernatant culture fluid.Meanwhile,14 strains of bacterium or eumycete were cultured in 21 cases of cloudy culture fluid,whose separation rate was 66.7%.In interference test bacterium was significantly inhibited when the bacterial density〈10^4/ml,and the solutions were different degree cloudy that the inhibition was weak at high density（≥10^4/ml）,so if urea and arginine decomposition test at least one positive bacteria,can lead to a false-positive.Conclusion Mycoplasma solution can not inhibit the growth of bacterium with the existence of great amount bacterium,which could decompose urea or arginine,and the mycoplasma test results could be false-positive.So,the proper result of mycoplasma tests can not be judged only by the color change of solution,other examinations and clinical symptoms also should be taken into consideration.

关 键 词：解脲支原体 人型支原体 鉴定与药敏 假阳性 

分 类 号：R446.6[医药卫生—诊断学]