pH胁迫对日本对虾非特异性免疫因子及RNA/DNA比值的影响  

Effects of pH stress on non-specific immune factors and RNA/DNA ratio of Marsupenaeus japonicus

作　　者：赵先银[1,2] 李健[2] 李吉涛[2] 何玉英[2] 张喆[2] 常志强[2] 

机构地区：[1]上海海洋大学,201306 [2]农业部海洋渔业资源可持续利用重点开放实验室中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《渔业科学进展》2011年第1期60-66,共7页Progress in Fishery Sciences

基　　金：公益性农业行业科研专项(200803012);国家虾产业技术体系(nycytx-46);国家863计划项目(2010AA10A401)共同资助

摘　　要：研究了pH胁迫对日本对虾血清非特异性免疫因子及对虾肌肉RNA/DNA比值的影响。结果表明,低pH胁迫组(pH 7.2)和高pH胁迫组(pH 9.2)总一氧化氮合成酶(TNOS)活力分别在31、2 h时达到最大;而诱导型一氧化氮合成酶(INOS)活力在3 h时达到最大值,随着胁迫时间的延长,酶活力逐渐降低,至72 h趋于稳定,并表现出高pH变化免疫适应能力较差的现象。两pH胁迫组酚氧化酶(PO)活力呈现峰值变化,在12 h时达到最大值,之后逐渐降低,至72 h后趋于稳定。溶菌酶(LZM)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-PX)活力随着pH胁迫时间的增加而降低,同样表现出高pH变化免疫适应能力较差的现象。另外,pH胁迫条件下日本对虾的肌肉RNA/DNA比值显著低于正常对照组日本对虾肌肉的RNA/DNA比值,这可能是由于pH胁迫影响了对虾体内的物质代谢所致。The effects of pH stress on non-specific immune factors and the RNA/DNA ratio of Marsupenaeus japonicus were investigated.It was found that the activity of total nitric oxide synthase（TNOS） in M.japonicus of low pH group（pH 7.2） and high pH group（pH 9.2） reached the maximum at 3h and 12h,respectively.The activity of inducible nitric oxide synthase（INOS） in pH stress groups reached the maximum at 3h,then gradually declined and stabilized at 72h.The activity changes of phenoloxidase（PO） in M.japonicus under both the low and high pH conditions showed that they reached the peak value at 12h,gradually decreased thereafter,and became stable after 72h.Activities of lysozyme,superoxide dismutase,and glutathione peroxidase in M.japonicus decreased gradually under pH stress.The changes of non-specific immune enzymatic activity showed that the immune adaptive activity in M.japonicus exposed to the high pH stress was lower than those exposed to the low pH stress.Moreover,the RNA/DNA ratio in the muscle of M.japonicus under the pH stress conditions was significantly lower than that of the control group,supposedly due to the effects of pH stress on metabolism of M.japonicus.

关 键 词：PH胁迫 日本对虾 非特异性免疫因子 RNA/DNA 

分 类 号：S917.4[农业科学—水产科学]