凡纳滨对虾类泛素折叠修饰蛋白Ufm1基因克隆、序列分析及结构预测  

Cloning and sequencing of ubiquitin-fold modifier 1(Ufm1) gene and its structure prediction in Litopenaeus vannamei

作　　者：盛小伟[1,2] 高永华[1,2] 彭金霞[1] 李咏梅[1] 陈晓汉[1] 熊建华[1] 

机构地区：[1]广西水产研究所南美白对虾遗传育种中心,南宁530021 [2]桂林理工大学化学与生物工程学院,广西桂林541000

出　　处：《南方农业学报》2011年第2期192-196,共5页Journal of Southern Agriculture

基　　金：国家科技支撑计划项目(2008BADB9B03);国家虾产业技术体系岗位科学家经费项目(nycytx-46);广西直属公益性科研院所基本科研业务项目(2060302GXIF-2008-02)

摘　　要：【目的】了解类泛素折叠修饰蛋白(Ufm1)的结构特征和蛋白功能,为进一步探究Ufm1基因功能提供参考。【方法】以同一生长性状分离凡纳滨对虾家系的肌肉组织为材料,构建凡纳滨对虾生长性状差减cDNA文库,通过斑点杂交筛选获得Ufm1基因,经全长cDNA文库筛选获得Ufm1基因全长序列。【结果】Ufm1基因全长cDNA序列长714bp,包含261bp的开放阅读框,推测编码的蛋白质为86个氨基酸,预测的分子量为9.3ku,等电点pI为6.55;其编码蛋白无信号肽,无跨膜区域,可能定位于细胞质中,属于亲水性蛋白,含有1个酪蛋白激酶II磷酸化位点、1个N-糖基化位点、2个蛋白激酶C磷酸化位点;序列同源性分析表明,不同进化地位物种的Ufm1基因存在较高的同源性。【结论】Ufm1基因在系统进化上高度保守,这为进一步探究Ufm1基因功能及原核表达等奠定了基础。[Objective]The present experiment was conducted to analyze the structural characteristics and protein function of ubiquitin-fold modifier 1 （Ufml） gene. [Method]The muscular tissue of Litopenaeus vannamei family with growth character segregation was used to construct the subtracted cDNA libraries of growth traits in Litopenaeus vannamei and obtain Ufml gene using dot blot technique. The full length of Ufml gene sequence in Litopenaeus vannamei was screened from a full length cDNA library. [Resuh]The full length of eDNA sequence of Ufml gene was 714 bp, and contained a 261 bp open reading frame （ORF） encoding 86 amino acids （aa）. The predicted molecular weight was 9.3 ku and the theoretical isoelectric point was 6.55. The bioinformatics analysis results revealed that the predicted encoding protein had no signal peptide and notable transmembrane region. It may be located in the cytoplasm and belonged to the hydrophilia protein. The protein contained a casein kinase II phosphorylation loci, a N-glycosylation loci and two protein kinase C phosphorylation loci. The deduced amino acid sequence analysis results showed that Ufml genes in different species had high homology as also revealed by phylogenic tree.

关 键 词：凡纳滨对虾 差减CDNA文库 Ufm1基因 生物信息学 

分 类 号：S945.1[农业科学—水产养殖]