渗透胁迫下群众杨根细胞中的K^+累积效应  

K^+ Accumulation in Poplar Roots Induced by Osmotic Stress

作　　者：周晓阳[1] 张辉[1] 夏新莉[1] 

机构地区：[1]北京林业大学生物学院,北京100083

出　　处：《林业科学研究》1999年第4期346-349,共4页Forest Research

基　　金：1996~ 1998年国家自然科学基金资助课题"干旱胁迫下杨树气孔运动机制研究"的部分内容

摘　　要：群众杨插条苗经 PEG渗透胁迫处理后 ,取根部样品 ,经过快速冷冻、冷冻干燥、乙醚真空渗透及塑料包埋后 ,用透射电镜 X-射线能谱微区分析技术 ,对根表皮层、皮层及中柱薄壁组织细胞的细胞壁、细胞质和液泡中的 K+含量与分布进行了测定。结果表明 ,在渗透胁迫下 ,在根各部位组织细胞的细胞壁、细胞质和液泡中 ,K+含量都有不同程度的增加 ,增加的 K+主要积累在液泡中。渗透胁迫所促进的 K+吸收可被蛋白质合成抑制剂环己酰亚胺所抑制。Cuttings of Populus × xiaozhuanica W.Y.Hsu et Liang cv.‘ Popularis ’ 35 44 were treated with PEG solution. After fast freezing,freeze drying,pressure infiltration with ether and embedding in plastic,K + content were measured by X ray microanalysis in cell wall,cytoplasm and vacuole of rhizodermis, cortex and stelar parenchyma cells of roots. Compared to the controls,K + content in all root tissues were increased by osmotic stress.In particular,the increasing K + was largely accumulated in vacuole. The K + accumulation induced by osmotic stress could be inhibited completely by cycloheximide.

关 键 词：群众杨 根细胞 K^+累积 渗透胁迫 微区分析 X线 

分 类 号：S792.119[农业科学—林木遗传育种]