RP-HPLC法测定不同产地杜仲皮中桃叶珊瑚苷的含量  

Determination of Aucubin in Eucommia ulmoides bark from different origin by RP-HPLC

作　　者：姜晓芳[1] 张翠利[2] 李钦[1] 杜红岩[3] 杜兰英[3] 付建敏[3] 

机构地区：[1]河南大学中药研究所,河南开封475004 [2]黄河科技学院医学院,河南郑州450006 [3]中国林业科学研究院经济林研究开发中心,河南郑州450003

出　　处：《河南大学学报（医学版）》2011年第1期14-16,共3页Journal of Henan University:Medical Science

基　　金：国家林业公益性行业专项(201004029);国家"948"项目(2008-4-66);河南省科技攻关重点项目(102102310188);河南省教育厅自然科学基金(2008A360003)

摘　　要：目的：测定不同产地杜仲皮中桃叶珊瑚苷的含量。方法：采用高效液相色谱法,采用Diamonsil C18色谱柱（5μm,150 mm×4.6 mm）;流动相：甲醇-水（6∶94）;柱温：15℃;流速：1 mL/min;检测波长：206 nm。结果：桃叶珊瑚苷在0.165 6~3.312μg范围内线性良好。平均加样回收率为99.24%,RSD为1.74%。结论：本法快速、准确,可以用于桃叶珊瑚苷的含量测定。Objective：To determine the contents of aucubin in Eucommia ulmoides bark from different origin.Methods：The quantitative analysis was carried out with RP-HPLC on a Diamonsil C18 column（5 μm,150 mm×4.6 mm） with temperature at 15 ℃,the mobile phase was methanol-water（6：94） at 1 mL /min,and detection wavelength was 206 nm.Results： The linear ranges were 0.1656～3.312μg,The average recovery were 99.24 %,the relative standard deviations（RSD） were 1.74%.Conclusion： The method is proved to be specific and accurate.It can be used to determine the contents of aucubin in Eucommia ulmoides bark from different origin.

关 键 词：高效液相色谱法 桃叶珊瑚苷 含量测定 

分 类 号：R927.2[医药卫生—药学]