重组质粒pCKM—mPTH对甲状旁腺功能低下症动物模型血钙的影响  

Influence of recombinant plasmid pCKM-mPTH to the serum calcium concentration of the animal models of hypoparathyroidism

作　　者：梁月强 刘大钺[2] 曹劲 朱易凡[2] 李江波 林景能 

机构地区：[1]高要市人民医院,526020 [2]中山大学附属第一医院甲状腺外科,广州510080 [3]Houston, The Center for Environmental and Genetic Medicine,Alkek Institute of Biosciences and Technology, Texas A＆M University System Health Science Center

出　　处：《国际医药卫生导报》2011年第6期657-660,共4页International Medicine and Health Guidance News

基　　金：中山大学青年教师基金资助项目（3171911）

摘　　要：目的研究重组pCKM—mPTH质粒在SD大鼠甲状旁腺功能低下症模型治疗中对血钙浓度的影响。方法将60只SD大鼠制作甲状旁腺功能低下症模型后，随机分为3组，分别肌注pcDNA3．1（＋）空质粒、mPTH质粒和pCKM—mPTH质粒，不同时间用放免法测定血清甲状旁腺素（PTH）浓度，采用MTB比色法检测血清钙浓度。结果动物模型在注射mPTH和pCKM～mPTH质粒后，其血清PTH和钙浓度均出现显著升高，之后出现回落，但pCKM—mPTH质粒组回落较缓慢，其作用维持近30天。结论质粒pCKM—mPTH在SD大鼠体内合成的甲状旁腺素能有效的调节血清钙的浓度，且转染效率和维持时间优于不含pCKM启动子的mPTH质粒。Objective To study the influence of recombinant plasmid pCKM-mPTH to the serum calcium concentration of the SD rats ofhypoparathyroidism model. Methods 60 SD rats of hypoparathyroidism models were randomly divided into three groups, which were injected intramuscular pcDNA3.1 （ ＋ ） empty vector, mPFH plasmids and pCKM-mPTH plasmid via muscle. At different times, rats were measured serum parathyroid hormone （ PTH ） concentration using radioimmunoassay, and serum calcium concentration using MTB colorimetric assay. Results After being injected with mPTH and pCKM-mPTH plasmid, serum PTH and calcium appeared significantly increased and fell later, but that of pCKM-mPTH plasmid groups fell more slowly, which continued nearly 30 days. Conclusion Recombinant plasmid pCKM-mPTH can effectively regulate the concentration of serum calcium after transfecting SD rats of hypoparathyroidism model. Furthermore, its transfection efficiency and the maintenance of time are superior to non-pCKM promoter mPTH plasmid.

关 键 词：甲状旁腺功能低下症 基因重组 基因治疗 血清钙 甲状旁腺素 

分 类 号：R582.2[医药卫生—内分泌]