功能纳米颗粒对木瓜蛋白酶吸附动力学及热力学研究  

Adsorption Kinetics and Thermodynamics of Papain on Functional Nanoparticles

作　　者：宋明敏[1] 聂华丽[1] 朱利民[1] 

机构地区：[1]东华大学化学化工与生物工程学院,上海201620

出　　处：《化学世界》2011年第3期146-149,154,共5页Chemical World

基　　金：国家自然科学基金资助项目(50773009)

摘　　要：以磁性纳米颗粒(MNPs)为内核,外层包裹壳聚糖(CS),进一步键和α-酮戊二酸(α-KA),制备出一种新型的磁性离子交换色谱介质(α-KA-CS-MNPs)。分别在277,298,310K的均衡条件下,批量法对木瓜蛋白酶进行等温吸附测定,实验所得数据分别用拟一级动力学模型和拟二级动力学模型进行分析。结果表明:拟二级动力学吸附模型具有更好的线性相关性;磁性复合纳米颗粒对木瓜蛋白酶的吸附更符合Freundlich模型的吸附行为;通过对吸附热力学参数△H°、△S°及△G°的分析,表明吸附过程是自发进行且伴随着吸热及熵值的增加,吸附趋势随着温度的升高而有所增大。A new type of magnetic ion-exchange chromatogram medium coating with chitosan was prepared with α-ketoglutaric acid as ligand and magnetic nanoparticle as a core.Papain isotherm data were determined by using batch method at 277,298,310 K under equilibrium.The obtained experimental data were analyzed with the Lagergren pseudo-first-order model and the pseudo-second-order kinetics.The results indicated that the pseudo-second-order kinetic model provided a better correlation of the experimental results and the Freundlich isotherm could describe the adsorption behavior of papain on magnetic composite nanoparticles.Various thermodynamic parameters,△H°,△S° and △G°,were analysed,and the results showed that the adsorption process was spontaneous and endothermic,and accompanied with entropy increases.The adsorption was increased with temperature.

关 键 词：功能性纳米颗粒 Α-酮戊二酸 动力学 热力学 

分 类 号：O647.32[理学—物理化学]