石斛兰“绿色星辰×出水芙蓉”杂交种子无菌萌发与组培快繁研究  

Sterile Germination of the Hybrid Seeds of [Den.Burana Green Star × Den.Rainbow-compactum] and Rapid Propagation of F_1 Plantlets via Tissue Culture

作　　者：符岸军[1] 李娟玲[2] 李劲松[1] 张勇[2] 刘国民[2] 

机构地区：[1]海南省三亚市南繁科学技术研究院,海南三亚572000 [2]海南大学苦丁茶研究所,海南海口570228

出　　处：《安徽农业科学》2011年第5期2577-2580,共4页Journal of Anhui Agricultural Sciences

基　　金：国家科技基础条件平台建设子项目(2005DKA21006);国家科技支撑计划子项目(2007BAD45B06)

摘　　要：[目的]研究石斛兰"绿色星辰×出水芙蓉"杂交种子无菌萌发与组培快繁。[方法]以"绿色星辰"为母本、"出水芙蓉"为父本进行杂交,并对其杂交种子进行了离体培养和组培快繁。[结果]石斛兰杂交种"绿色星辰×出水芙蓉"的F1代种子在改良Knudson培养基上(附加1.00 mg/L 6-BA、1.00 mg/L NAA和10.0%熟香蕉泥)可获得理想的无菌萌发效果,且原球茎在该培养基上也能较快增殖。以1/2MS最适合于该杂种石斛兰F1代无菌实生苗的组培快繁;8种供试培养基用于增殖培养的效果为:1/2MS>MS>1/3MS>1/4MS≈N6>改良Knudson≈B5>H。NAA用于杂种石斛兰"绿色星辰×出水芙蓉"F1代无菌实生苗的生根壮苗效果要优于IBA;NAA的推荐浓度为2.00 mg/L。[结论]为今后的新品种选育提供了育种材料。[Objective] This study was to germinate the hybrid seeds of [Den.Burana Green Star × Den.Rainbow-compactum] under aseptic condition and to explore the parameters for rapid propagation of F1 plantlets via tissue culture.[Method] Hybridization between Den.Burana Green Star（female parent） and Den.Rainbow-compactum（male parent） was performed and the in vitro culture and proliferation of F1 hybrids were studied using eight different basic media including MS,1/2MS,1/3MS,1/4MS,B5,N6,modified Knudson and H.[Result] Improved Knudson medium appended with 1.00 mg/L 6-BA,1.00 mg/L NAA and 10% mature banana puree performed best in F1 seed germination under aseptic condition,as well as the rapid propagation of protocorm-like body.Of all the eight media tested,1/2MS is the medium most suitable for the in vitro rapid propagation of the F1 seedlings.Efficiency of eight media in the in vitro rapid propagation was in order 1/2MS MS1/3MS1/4MS ≈N6 improved Knudson ≈B5H.NAA presented better rooting and growth-promoting effect in the in vitro rapid propagation of the F1 seedlings than IBA.And the optimal NAA concentration to recommend from our experiment results was 2.00 mg/L.[Conclusion] Our experimental results provided mature method and important technological information for hybrid breeding of dendrobium.

关 键 词：石解兰 杂种 无菌萌发 组培快繁 

分 类 号：S682.31[农业科学—观赏园艺]