植物生长调节剂TDZ在草莓花药培养中的应用  

Application of Plant Growth Regulator TDZ in Strawberry Anther Culture

作　　者：王敬东[1] 张丽[1] 马洪爱[1] 陈晓军[1] 宋玉霞[1] 

机构地区：[1]宁夏农林科学院农业生物技术研究中心,宁夏银川750002

出　　处：《安徽农业科学》2011年第5期2588-2590,2622,共4页Journal of Anhui Agricultural Sciences

基　　金：宁夏回族自治区科技攻关项目(KGZ-16-07-02);北京林业大学林木育种国家工程实验室开放课题

摘　　要：[目的]探讨植物生长调节剂TDE在草莓花药培养中的应用。[方法]采用不同浓度TDZ与NAA植物生长素进行组合,观察其对草莓愈伤组织诱导及其分化的影响。[结果]MS+TDZ 1.0 mg/L+NAA 0.5 mg/L适用于草莓愈伤组织的分化,分化率高达75%,但TDZ对草莓愈伤组织的诱导无显著作用。[结论]该研究结果为草莓通过花药培养途径进行规模生产提供了参考。[Objective] The aim was to investigate the application of plant growth regulator thidiazuron（TDZ） in strawberry anther culture.[Method] Different combinations of TDZ and naphthalene acetic acid（NAA） auxine with different concentrations were used for induction culture,and then its effect on callus induction and plant regeneration were observed.[Result] The combination of 1.0 mg/L TDZ＋0.5 mg/L NAA was effective to accelerating the differentiation and regeneration of strawberries,and the differentiation ratio was as high as 75%.However,TDZ showed insignificant effect on strawberries anther induction.[Conclusion] This research had provided reference for large-scale production of strawberry through anther culture.

关 键 词：草莓花药 TDZ 愈伤组织 不定芽 

分 类 号：S482.8[农业科学—农药学]