棉花开花前不同天数胚珠的采集标准检测  

Determination of Collection Standard of Cotton Ovlues on Different Days before Flowering

作　　者：温玮[1] 陈蔷[1] 孙海彦[1] 崔百明[2] 彭明[1,2] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]新疆石河子大学生命科学学院,新疆石河子832000

出　　处：《安徽农业科学》2011年第5期2611-2612,共2页Journal of Anhui Agricultural Sciences

基　　金：国家"973"计划重点项目(2004CB117307);农业部重大转基因专项项目(2009ZX08005-027B);兵团博士资金项目(2007JC08)

摘　　要：[目的]检测棉花开花前不同天数胚珠的采集标准,以期更清楚地研究棉花开花前后胚珠中基因的表达变化。[方法]采用花苞长、花苞宽、萼片长和萼片宽4种不同的参数来标准化陆地棉新陆早13号开花前不同天数棉花胚珠的特征。[结果]只有花苞长随花期的临近逐渐增长,而其他参数均没有明显变化。因此,选择花苞长为检测开花前不同天数胚珠的标准。统计分析表明,确定以1.0~1.2cm长的花苞为标准采集开花前5 d的胚珠,以1.3~1.5 cm长的花苞为标准采集开花前3 d的胚珠,以1.7 cm以上的花苞为标准采集开花前1 d的胚珠。[结论]该研究结果为后期试验中开花前胚珠样品的采集提供参考数据,也为更精确检测开花前后胚珠中基因表达的变化奠定了基础。[Objective] The aim was to determine the collection standard of cotton ovules on different days before flowering,and then to more clearly study the changes in gene expression of cotton ovules before and after blooming.[Method] The standard to distinguish ovules in Xinluzao13 on different days before flowering was defined by the length and width of alabastrum and sepal.[Result] The alabastrum length increased gradually with approaching of flowering time,while other parameters did not show the same tendency.Therefore,the alabastrum length was chosen as the standard to determine the ovules on different days before folwering.The results of statistics analysis revealed that the alabastrums with length of 1.0-1.2 cm,1.3-1.5 cm and more than 1.7 cm respectively were determined as the standards for ovules collection on 5 d,3 d and 1 d before flowering.[Conclusion] This study will provide not only the reference data for the collection of ovules sample in the following experiment,but also the basis for more precisely detecting the changes in gene expression of ovules before and after flowering.

关 键 词：棉花 开花前胚珠 花苞 萼片 

分 类 号：S188[农业科学—农业基础科学]