油菜菌核病生防放线菌的分离与筛选  

Isolation and Screening of Biocontrol Actinomyces Against Sclerotinia Sclerotiorum

作　　者：韩立荣[1] 孙平平[1] 张宁[1] 冯俊涛[1] 张兴[1] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心陕西省生物农药工程技术研究中心,陕西杨凌712100

出　　处：《安徽农业科学》2011年第5期2668-2670,共3页Journal of Anhui Agricultural Sciences

基　　金：国家公益性行业(农业)科研专项(200903052)

摘　　要：[目的]分离筛选出对油菜菌核病菌具有较好防治效果的生防放线菌。[方法]采用平板稀释法从采集土样中分离纯化放线菌,以油菜菌核菌为靶标菌,通过生长速率法、菌核萌发试验和盆栽试验,筛选对其具有高效抑菌活性的放线菌。[结果]从采集土样中共分离纯化到132株放线菌,其中有35株对油菜菌核菌菌丝生长抑制率大于60%,占总分离菌株的26.5%。所分离的35株放线菌中有11株对菌核萌发抑制率在70%以上,其中菌株GSA22-4、GSA26-2、HVA25-3、HVA36-4和AXW4-1可完全抑制菌核的萌发。盆栽试验结果表明,有6株放线菌对油菜菌核病的防治效果在60%以上,其中放线菌株X1-8的防治效果达91.7%。[结论]该研究初步筛选出对油菜菌核病有良好生防效果的放线菌,为进一步的研究工作奠定了基础。[Objective] The research aimed to isolate and screen for Actinomyces with the bioactive activity against Sclerotinia sclerotiorum.[Method] Actinomyces were isolated and purified from the soil samples by the diluting plate method,and actinomyces with the high bioactivities against S.sclerotiorum were screened by the hyphae growth method,sclerotium germination inhibition test and pot trails in turn.[Result] 132 Actinomyces were isolated from the collected soil samples.The inhibition ratio of 35 Actinomyces on Sclerotinia sclerotiorum hyphae growth was higher than 60%,which was 26.5% of all the strains.11 of these 35 strains had the sclerotium germination inhibition ratio higher than 70%.The stains GSA22-4,GSA26-2,HVA25-3,HVA36-4 and AXW4-1 could completely inhibit the germination of sclerotium.Pot trails showed that 6 Actinomyces possessed the control effects on Sclerotinia sclerotiorum higher than 60%,and the inhibit ratio of stain X1-8 reached 91.7%.[Conclusion] The research initially screened Actinomyces with the bioactivity against Sclerotinia sclerotiorum and laid the foundation for the further research.

关 键 词：油菜菌核病 放线菌 筛选 防治效果 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]