山梨醇对水稻体细胞培养的特殊作用  

Special Functions of Sorbitol on Rice Somatic Cell Culture

作　　者：黄慧君[1] 黄道强[1] 

机构地区：[1]广东省农业科学院水稻研究所,广东广州510640

出　　处：《中国水稻科学》1999年第4期248-250,共3页Chinese Journal of Rice Science

摘　　要：山梨醇和甘露醇作碳源，对籼稻品系ＴＣ９０及其花培单倍体幼穗愈伤组织诱导、植株分化与染色体自然加倍的研究结果表明，２％和４％的山梨醇作唯一碳源时，可维持外植体生存２５ｄ以上，绿茵再生频率分别为５２．６％和９０．０％，单倍体自然加倍率达３０．５％。２％或４％的甘露醇作碳源时，外植体均不能生长，逐渐变得苍白、干瘪，培养７ｄ后失去分化再生能力。The effects of sorbitol and mannitol as carbon source on callus induction, plantlet regeneration and haploidchromosome doubling in diploid and haploid young panicle culture of indica rice line TC90 were studied. The results showedthat 2% or 4% sorbitol as medium carbon source could efficiently maintain explants alive at least for 25 days, and plantlet regeneration frequency reached 52. 6% and 90. 0% respectively, and haploid doubling rate reached as high as 30. 5%. On thecontrary, the explants did not produce any callus, became pale, shrunk and died out after being cultured seven days when 2%or 4% mannitol used as carbon source.

关 键 词：山梨醇 自然加倍 体细胞培养 水稻 

分 类 号：Q943.1[生物学—植物学] S511.035.3[农业科学—作物学]