肾移植受者外周血辅助性T淋巴细胞17、调节性T细胞表达及其平衡关系的研究  

Study on balance of T helper 17 and Treg cells in peripheral blood of renal allograft recipients

作　　者：眭维国[1] 李凤艳[2] 车文体[1] 陈结晶[1] 黄河[1] 戴勇[1] 

机构地区：[1]中国人民解放军第181医院肾脏科广西代谢性疾病研究重点实验室,桂林541002 [2]广西师范大学生命科学学院

出　　处：《器官移植》2011年第2期94-98,共5页Organ Transplantation

基　　金：广西省自然科学基金(0832289)

摘　　要：目的探讨肾移植慢性排斥反应(CR)患者外周血辅助性T淋巴细胞(Thelper,Th)17、调节性T细胞(regulatoryTcells,Treg)、Th17/Treg变化及意义。方法 采用流式细胞术检测24例肾移植CR患者(CR组)和22名健康志愿者(正常对照组)外周血中的Th17占CD4+T淋巴细胞的比例(Th17/CD4+)和CD4+CD25+CD127-Treg细胞占CD4+T淋巴细胞的比例(Treg/CD4+),以及计算Th17/Treg的比值,并作比较。结果 CR组外周血中Th17/CD4+为0～3.3%(中位数0.4%),明显低于正常对照组的0～4.0%(中位数1.65%)(P<0.01)。CR组和正常对照组的外周血Treg/CD4+分别为(11.7±4.3)%、(8.0±2.6)%,CR组明显高于正常对照组(P<0.01)。CR组外周血中Th17/Treg的比值为0～0.51%(中位数0.03%),明显低于正常对照组的0～0.83%(中位数0.21)%(P<0.01)。结论 肾移植受者外周血中存在Th17/Treg失衡,Th17/Treg失衡可能参与了肾移植的免疫异常从而导致CR的发生和发展。Objective To investigate the alteration of T helper（Th） 17 cells,regulatory T cells（Treg） and Th17/Treg in peripheral blood of renal allograft recipients with chronic rejection（CR）.Methods The ratios of Th17 cells and CD4＋CD25＋CD127-Treg cells in CD4＋T lymphocyte of peripheral blood were detected by flow cytometry in 24 renal allograft recipients with CR（CR group） and 22 healthy blood donors（normal control group）.The ratio of Th17/Treg was also calculated.Results The ratio of Th17/CD4＋ was 0-3.3 %（with a median of 0.4 %） in CR group,which was significantly less than 0-4.0 %（with a median of 1.65 %） in normal control group（P0.01）.The ratios of Treg/CD4＋T cells in peripheral blood were respectively（11.7±4.3）% in CR group and（8.0±2.6）% in normal control group.The ratio in CR group was significantly higher than that in normal control group（P0.01）.The ratio of Th17/Treg was 0-0.51 %（with a median of 0.03 %） in CR group,which was significantly less than 0-0.83 %（with a median of 0.21 %） in normal control group（P0.01）.Conclusion Th17/Treg imbalance exists in the peripheral blood of renal allograft recipients.It may play a potential role in the dysimmunity in renal transplantation and lead to occurrence and development of CR.

关 键 词：肾移植 慢性排斥反应 辅助性T淋巴细胞17 调节性T细胞 流式细胞术 

分 类 号：R725.6[医药卫生—儿科]