小剂量内毒素预处理对肝脏缺血再灌注损伤的保护效应  

The protective effect of preconditioning with LPS on ischemia-reperfusion injury in rat liver

作　　者：张武山[1] 

机构地区：[1]太原市中心医院

出　　处：《山西临床医药》1999年第4期251-253,共3页Shanxi Clinical Medicine

摘　　要：要⑶目的⑶观察小剂量内毒素⒉ Ｌ Ｐ Ｓ⒕预处理对大鼠肝脏缺血再灌注损伤⒉ Ｉ／ Ｒ⒕模型⒚方法⑶小剂量内毒素预处理组于术前 ２４ｈ 给予 Ｌ Ｐ Ｓ⒙分别于复灌后 ６０ｍ ｉｎ⒙９０ｍ ｉｎ⒙１２０ｍ ｉｎ 测定血中肝功能酶活性及肝组织中肿瘤坏死因子⒉ Ｔ Ｎ Ｆα⒕及前列腺素 Ｅ１ ⒉ Ｐ Ｇ Ｅ１ ⒕含量⒙并与缺血再灌注组比较⒚结果⑶ Ｌ Ｐ Ｓ预处理组在各采样时点肝功能酶活性组织 Ｔ Ｎ Ｆα含量均显著低于 Ｉ／ Ｒ 组⒙⒉ Ｐ＜ ０．０５⒕⒚而组织中 Ｐ Ｇ Ｅ 含量则明显高于 Ｉ／ Ｒ组⒉ Ｐ＜ ０．０５⒕⒚结论⑶小剂量 Ｌ Ｐ Ｓ预处理可能系通过刺激巨噬细胞合成 Ｐ Ｇ Ｅ１ ⒙而对大鼠肝脏 Ｉ／ ＲObjective:To study the protective effect of preconditioning with LPS on ischemia-reperfusion injury in rat liver.Methods:LPS was injected 24 hours before operation,the serum GOT,GPT activity and tissue TNFα,PGE 1 content of LPS preconditioning group were detected and compared with that of I/R group at 60min,90min and 120min when reperfused.Results:the enzyme activity and TNFα are significantly lower in LPS preconditioning group than that in I/R group(P<0.05),however,the content of PGE 1 is higher in LPS group than in I/R group(P<0.05).Con clusion:LPS can stimulate the macrophage cell to produce PGE,Which led to the protective effect of preconditioning on I/R injury in rat liver.

关 键 词：内毒素预处理 再灌注损伤 肝缺血 剂量 

分 类 号：R657.305[医药卫生—外科学]