一株Class Ⅰ新城疫病毒分离株NP和P蛋白基因分子特征和遗传进化分析  

Molecular Characterization and Phylogenetic Analysis of NP and P Genes of a Class Ⅰ Newcastle Disease Virus

作　　者：陈云霞[1,2] 刘华雷[1] 郑东霞[1] 赵云玲[1] 黄伟坚[2] 王志亮[1] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]广西大学动物科学学院,广西南宁530005

出　　处：《中国动物检疫》2011年第3期37-40,共4页China Animal Health Inspection

基　　金：国家自然科学基金资助(30901079);农业部动物疫情监测与防治项目

摘　　要：本研究对2009年实验室保存的一株Class Ⅰ新城疫病毒分离株NDV09-056的NP和P基因进行了扩增和序列分析,对其分子特征和遗传进化关系进行了分析。结果表明该分离株NP蛋白基因全长为1746nt,共编码489aa,其N端1～401氨基酸相对比较保守,C端402～479氨基酸变化比较大;同源性分析表明本分离株的NP蛋白基因与Ⅰ类新城疫病毒代表毒株之间核苷酸的同源性为93.5%～98.5%,而与Ⅱ类新城疫病毒代表毒株的同源性较低,介于77.0%～79.2%。P蛋白主要氨基酸突变区集中在57～107aa和135～212aa两个区域,而N端和C端相对保守;同源性分析表明本分离株的P蛋白基因与Ⅰ类新城疫病毒代表毒株之间核苷酸的同源性为90.4%～95.3%,而与Ⅱ类新城疫病毒代表毒株的同源性较低,介于68.3%～72.5%。遗传进化分析表明本分离株与Ⅰ类基因3型新城疫病毒代表株NDV08-004的亲缘关系最近。The NP and P genes of a Newcastle disease virus （NDV） isolate NDV09-056, obtained from domestic duck in China in 2009, were amplified by RT-PCR and sequenced. The complete NP gene of NDV09-056 isolate was composed of 1746 nucleotides encoding 489 amino acids. Homology analysis of NP gene of the isolate revealed that the homology of nucleotides among class I NDV isolates were between 93.5%-98.5%, and showed relative lower homology between this isolate and the class II NDV strains, 77.0%-79.2%. The full length of P gene of the isolate was composed of 1463 nucleotides, encoding 399 amino acids. Homology analysis of the P gene of the isolate revealed that the homology of nucleotides among class I NDV isolates were between 90.4%-95.3%, and showed relatively lower homology between the isolate and the class II NDV strains, 68.3% -72.5%.Phylogenetic analysis based on the complete NP gene and P gene showed that the NDV09-056 isolate was grouped into genotype 3 in class I. The evolutionary rates of NP gene and P gene of class I was similar based on the homology analysis and phylogenetic analysis.

关 键 词：新城疫病毒 Ⅰ类 NP基因 P基因 

分 类 号：S852.659.5[农业科学—基础兽医学]