牛皮蝇幼虫抗原基因原核表达条件的优化  

Optimization on Prokaryotic Expression Conditions of Hypodermin Genes

作　　者：娜仁高娃[1] 呼和巴特尔[1] 王文龙[1] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018

出　　处：《动物医学进展》2011年第3期32-36,共5页Progress In Veterinary Medicine

基　　金：教育部"春晖计划"项目(Z2007-1-01011;Z2007-1-01018);内蒙古自然科学基金项目(2009BS0403);内蒙古农业大学博士科研启动金(BJ07-48)

摘　　要：分别对构建的3种牛皮蝇幼虫抗原(Hypodermin A,B,C)基因重组表达菌BL21(pETHA)、BL21(pETHB)、BL21(pETHC)在不同的诱导时间、诱导剂浓度和诱导温度等条件下的表达情况进行了研究。结果表明,重组表达菌BL21(pETHA)、BL21(pETHB)、BL21(pETHC)最佳诱导时间均为4 h,最佳诱导剂浓度均为0.05 mmol/L,最佳诱导温度分别为37、37、30℃。研究结果为进一步获得大量重组抗原用于牛皮蝇蛆病的血清学诊断和免疫防控研究提供了依据。To investigate the optimal condition for expression of three kinds of constructed Hypoderma lineatum larvae antigens,Hypodermin A,B,C,in E.coli BL21（DE3）,the protein expression level was evaluated using SDS-PAGE and BandScan 5.0 gel analysis software,respectively.Optimum conditions for expression,such as the induction time,induction concentration and the temperatures were determined.The results showed that the optimal induction time of recombinant BL21（pETHA）,BL21（pETHB） and BL21（pETHC） was 4 h,their optimal induction concentration was 0.05 mmol/L,and their optimal temperatures were 37 ℃,37 ℃ and 30 ℃,respectively.The optimal condition is established for producing a large number of recombinant antigens which can be used in serological and immunological techniques for the diagnosis of hypodermosis.

关 键 词：牛皮蝇蛆病 牛皮蝇幼虫抗原 原核表达 表达条件优化 

分 类 号：Q786[生物学—分子生物学]