湖北省部分肉鸡场致病性大肠埃希菌的分离鉴定及耐药性分析  

Isolation and Drug Resistance Analysis of Pathologic Escherichia coli from Broiler in Hubei Province

作　　者：罗玲[1] 杨峻[1] 艾地云[1] 王红琳[1] 罗青平[1] 温国元[1] 张蓉蓉[1] 廖永红[1] 张琳[1] 邵华斌[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所动物胚胎工程及分子育种湖北省重点实验室,湖北武汉430064

出　　处：《动物医学进展》2011年第3期128-131,共4页Progress In Veterinary Medicine

基　　金：现代农业产业技术体系建设专项资金项目(nycytx-42-G3-04);动物胚胎工程及分子育种湖北省重点实验室项目(2010ZD108)

摘　　要：对湖北省部分肉鸡场的大肠埃希菌病流行病学进行调查,从采集的50份发病及死亡鸡肝脏、心脏和气囊中共分离出21株大肠埃希菌,对其培养特性、形态特征、生化特性、致病性进行了研究,初步鉴定为大肠埃希菌。同时对分离的21株大肠埃希菌的O群抗原进行了血清型鉴定,其结果为10株O78、6株O18,2株O1,还有3株有待于进一步的血清型鉴定。动物试验表明,已鉴定出血清型的18株大肠埃希菌均有不同程度的致病性。选择临床上常用的15种药物进行药敏试验,结果显示鸡大肠埃希菌的耐药性已经到了非常严重的程度。Epidemiology of colibacillosis from broiler farms in part region of Hubei province were investigated.Twenty-one strains were isolated from liver,heart and air bladder of fifty sick and dead broilers.Based on the basis of culture characters,morphology observation,physiological and biochemistry tests,pathogenicity,the bacteria were identifed as O78（10 strains）,O18（6 strains）and O1（2 strains）.Three strains were not identifed and needed further serological study.Animal test results demonstrated that the identifed strains had different pathogenicity.The results of fifteen drug sensitive tests showed that drug resistance of broiler Escherichia coli became more and more severe now.

关 键 词：致病性大肠埃希菌 血清型 分离鉴定 耐药性 肉鸡 

分 类 号：S852.612[农业科学—基础兽医学]