响应面分析法在优化桃儿七鬼臼毒素CO_2超临界萃取工艺中的应用  

Application of supercritical carbon dioxide extraction of podophyllotoxin from Sinopodophyllum emodi with response surface methodology

作　　者：杜微[1] 李唯[1] 杨德龙[1] 栗孟飞[1] 杨青[1] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2011年第1期144-150,共7页Journal of Gansu Agricultural University

基　　金：高等学校博士学科点专项科研基金(2009620211006)

摘　　要：在单因素试验基础上,以萃取压力、萃取温度、萃取时间为影响因素,鬼臼毒素提取率为响应值,根据中心组合试验设计原理采用3因素3水平的响应面分析法,对桃儿七中鬼臼毒素的二氧化碳超临界萃取(CO2-SFE)条件进行优化.结果表明:CO2-SFE萃取桃儿七中鬼臼毒素的最佳工艺条件为:萃取压力38MPa,萃取温度50℃,萃取时间52min,在此条件下鬼臼毒素的提取率可达11.38%.The supercritical carbon dioxide extraction of podophyllotoxin from Sinopodophyllum emodi was optimized with the conditions of extraction pressure,temperature and time by response surface methodology（RSM） based on single factor experiments.According to Box-Benhnken principles of experimental design with 3 factors with 3 levels of response surface analysis to determine the process conditions on the extraction rate.The results showed that the optimum extraction conditions were as follows： pressure 38.89MPa,temperature 50.25 ℃,time 52.51 min.Under such conditions,the podophyllotoxin extraction rate reached 11.38 %.

关 键 词：桃儿七 鬼臼毒素 CO2-SFE萃取 响应面分析法 

分 类 号：R284.2[医药卫生—中药学]