熊果酸对人早幼粒白血病细胞的增殖抑制、周期阻滞及凋亡诱导作用  

Ursolic Acid Induced Growth Inhibition,Cell Cycle Arrest and Apoptosis in Human Promyelocytic Leukemia Cell Line HL-60 Cells

作　　者：杨邵华[1] 侯茜[1] 张琼[1] 刘国安[1] 丁兰[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《甘肃科学学报》2011年第1期67-71,共5页Journal of Gansu Sciences

基　　金：西北师范大学知识与科技创新工程项目(NWNU-KJCXGC-03-65)

摘　　要：利用台盼蓝排染法、AO/EB双染检测法和流式细胞术检测了熊果酸对人早幼粒白血病细胞HL-60的增殖、细胞凋亡和细胞周期的作用.结果表明,低浓度(15～20μmol/L)时药物对HL-60细胞的生长表现为抑制作用,随浓度升高(20～35μmol/L)出现致死效应,并呈现剂量依赖和时间依赖性;AO/EB双染分析表明,药物能够诱导细胞凋亡,随着药物浓度增加和处理时间延长,细胞凋亡率上升,并且伴有少量细胞坏死;流式细胞术检测表明,在20μmol/L时,熊果酸可引起明显的HL-60细胞S期阻滞,随着浓度升高,周期阻滞作用明显加强.细胞周期阻滞可能是药物抑制细胞增殖进而引起凋亡的原因.The effects of ursolic acid on proliferation,apoptosis and cell cycle on huinaia promyelocytic leukemia HL-60 cells were studied using typanblue exclusion method,AO/EB stain assay and cell lab quanta. The results showed that ursolic acid inhibited cell growth in low concentration （15-20μmol/L） and lethal effect in high concentration（20-35μmol/L） and presented in dose-dependent and time-dependent relations. The cells showed morphological characteristics of apoptosis after treated with the Chemical when stained with AO/EB. The apoptosis in time and dose-dependent patterns and few necrosis cells are observed at the same time. The result of flow cytometry exhibites ursolic acid induced S phrase arrest cell in HL-60 cells. The cell cycle arrest resulted from the chemical maybe the reason that causing cell growth inhibition and apoptosis.

关 键 词：熊果酸 生长抑制 周期阻滞 凋亡 

分 类 号：Q946[生物学—植物学] Q255