HPLC法测定紫杉醇血药浓度条件的筛选  

Screening the determining condition of HPLC for the concentration of paclitaxel in serum

作　　者：孟素蕊[1] 何景华[1] 焦建杰[1] 牛瑞芳[2] 高洋[1] 李芹[1] 韩晓文[1] 

机构地区：[1]天津医科大学药理学教研室,天津300070 [2]天津医科大学肿瘤医院,天津300060

出　　处：《天津医科大学学报》2011年第1期6-9,共4页Journal of Tianjin Medical University

基　　金：国家高技术研究发展计划(863计划)资助项目(2007AA021802)

摘　　要：目的:筛选小鼠血清中紫杉醇浓度测定的方法。方法:以Agilent C18柱为固定相,检测波长为227 nm,流速为1 mL.min-1,25℃条件下对流动相、内标、提取方法进行筛选。结果:确定以固相萃取方法提取样品,内标为地西泮和多西紫杉醇,流动相为磷酸水溶液-乙腈(50:50 V/V)以达到最佳的分离效果,测定线性范围在0.39～100.00μg.mL-1,回归方程为Y=13.325X+0.851 9(R2=0.999 3)。结论:筛选出的方法血清干扰少,样品测定周期短、快速、准确,为血清中紫杉醇浓度的测定提供了优选的方法。Objective： To screen a method for determining the concentration of paclitaxel in serum.Methods： The C18 column was used and the detection was carried out at 227 nm with UV detector.The mobile phase,internal standard and extraction method were optimized at 25 ℃.Results： Diazepam and taxotere was used as the internal standard,the solid phase extraction（SPE）were used to extract the sample,the mobile phase consisted of phosphoric acid： acetonitrile（50：50 V/V） delivered at a flow rate of 1 mL·min-1.The calibration curves were linear in the rang of 0.39~100.00 μg·mL-1 for paclitaxel.Regression equation：Y=13.325X＋0.851 9（R2=0.999 3）.Conclusion： This optimized method has less interference with serum.It＇s a rapid and accurate way to measure the concentration of paclitaxel in serum.

关 键 词：紫杉醇 RP-HPLC 血药浓度 筛选 

分 类 号：R92[医药卫生—药学]