糖尿病大鼠AGEs与nephrin表达的研究  

Research of AGEs and nephrin expression on diabetic rats

作　　者：范振迁[1] 孔艳[1] 付建如[1] 韩荣凤[1] 郑少雄[1] 

机构地区：[1]天津医科大学第二医院内分泌科,天津300211

出　　处：《天津医科大学学报》2011年第1期21-23,30,共4页Journal of Tianjin Medical University

摘　　要：目的:探讨终末期糖基化产物(AGEs)、肾病蛋白(nephrin)在糖尿病大鼠肾脏组织中的表达及其在糖尿病肾病发生、发展中的作用。方法:用免疫组化方法检测链脲佐菌素诱发糖尿病及正常对照SD大鼠肾脏中AGEs、nephrin的表达,并计算其阳性指数,同时测定各组大鼠24 h尿蛋白定量及肾功能等一般生化指标,并对大鼠肾脏AGEs、nephrin与蛋白尿进行相关分析。结果:与对照组相比,糖尿病组大鼠肾小球上AGEs表达明显增加(P<0.01),nephrin表达明显减少(P<0.01),24 h尿蛋白排泄量明显增加(P<0.01),大鼠肾小球AGEs的表达和nephrin的表达呈负相关(r=-0.921,P<0.01);nephrin蛋白表达与24 h尿蛋白排泄量呈负相关(r=-0.904,P<0.01);AGEs表达与24 h尿蛋白排泄量呈正相关(r=0.974,P<0.01)。结论:AGEs可能介导了nephrin的表达缺失,进而导致足细胞损伤并加速了糖尿病肾病的进展。Objective： To study the expression of advanced glycation end products（AGEs）and nephrin on diabetic rat kidney and their roles in the development of diabetic nephropathy.Methods：AGEs and nephrin expression were detected by immunohistochemistry on streptozotocin induced diabetic and normal SD rat,and the positive rate was calculated with semi-quantitative method,24 h microalbuminuria were measured quantitatively with the biuret method.The correlation of proteinuria and expression of AGEs and nephrin were analyzed.Results： Compared with the control group,the expression of the AGEs on diabetic rat glomerular increased significantly（P0.01）,nephrin expression was significantly decreased（P0.01）,24 h microalbuminuria in diabetic group increased significantly（P0.01）;expression of AGEs and nephrin on glomerular was negatively correlated（r=-0.921,P0.01）;24 h microalbuminuria and nephrin expression was negatively correlated（r =-0.904,P0.01）;and the expression of AGEs positively correlated with 24 h microalbuminuria（r=0.974,P0.01）.Conclusion： AGEs may mediate the loss of nephrin expression,leading to podocyte injury and the progression of diabetic nephropathy.

关 键 词：足细胞 肾小管 AGES NEPHRIN 糖尿病肾病 蛋白尿 大鼠 

分 类 号：R587.2[医药卫生—内分泌]