利用转基因番茄生产葡激酶的研究  

Producing Staphylokinase with Transgenic Tomato

作　　者：杜景川[1] 梁宏伟[1,2] 陆海[1] 陈发菊[2] 李凤兰[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]三峡大学生物技术研究中心,湖北宜昌443002

出　　处：《福建林业科技》2011年第1期32-36,共5页Journal of Fujian Forestry Science and Technology

基　　金：国家林业局948项目(2006-4-73)

摘　　要：利用农杆菌介导的方法,将葡激酶(Staphylokinase,SAK)基因导入番茄中。经PCR、Southern杂交和Northern杂交检测,葡激酶基因已整合到再生番茄植株基因组中,共获得8个转基因株系。经ELISA检测,转基因番茄的果实和叶片均能表达SAK蛋白,SAK蛋白在果实和叶片可溶性蛋白中的比例最高分别为3.42%和2.47%。转基因番茄中的SAK蛋白具有一定的溶栓活性,溶栓比活力为3 866 AU·mg-1。In this study, we introduced SAK into tomato via Agrobacterium tumefaciens-mediated transformation. Staphylokinase gene had been integrated into the genome of regenerated tomato plants by PCR, Southern crossbreeding and Northern crossbreeding, 8 transgenic tomato lines were indentified. Through ELISA test, transgenic tomato fruits and leaves could show SAK protein. The SAK protein content in transgenic tomato fruits and leaves reached levels of 3.42% and 2.47%. SAK protein possessed a certain of fibrinolytic activity in transgenic tomato, fibrinolytic activity was 3 866 AU/mg.

关 键 词：葡激酶 番茄 转基因 生物反应器 

分 类 号：Q943.2[生物学—植物学] S641.2[农业科学—蔬菜学]