番茄茎叶提取物对萝卜蚜的毒力及其活性物质的分离纯化和结构的初步解析  

Control Effect of Leaf and Stalk Extracts of Lycopersicon esculentum Mill on Lipaphis erysimi Kaltenbach and Isolation,Purification and Structure Identification of the Active Substances

作　　者：李会[1] 吴世标[2] 贾福丽[1] 陈义娟[1] 胡金锋[2] 吴节莉[3] 代光辉[1] 

机构地区：[1]上海交通大学农业与生物学院农业部都市农业(南方)开放重点实验室,上海200240 [2]华东师范大学脑功能基因组学研究所,上海200062 [3]上海交通大学分析测试中心,上海200240

出　　处：《上海交通大学学报（农业科学版）》2011年第1期43-47,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家科技部农业科技成果转化基金(083919n8100);上海市蔬菜学重点学科基金(B209);上海农乐生物制品股份有限公司研究与发展基金资助

摘　　要：番茄(Lycopersicon esculentum Mill)茎叶提取物对萝卜蚜(Lipaphis erysimi Kaltenbach)具有防治效果,且随着浓度的提高活性加强。在田间小区试验中,当浓度为30 mg.mL-1时,对萝卜蚜的防效为97%。室内生测实验结果表明,其对萝卜蚜毒效的LC50为10.36 mg.mL-1。通过对番茄茎叶进行提取、分离和纯化,结合活性跟踪,经红外光谱、紫外光谱、质谱、核磁共振等分析,初步确定该活性结构为一分子量为300.1415的脂肪酸酯类化合物。This study showed that extracts from stalks and leaves of tomato plant（Lycopersicon esculentum Mill） had a control effect against Turnip aphid（Lipaphis erysimi Kaltenbach）.The efficiency increased as the dose increased.The control effect（97%） of the extracts（30 mg·mL-1） against the insect was demonstrated through field experiment.The indoor bioassay results also showed that the extracts are toxic to turnip aphid with LC50 of 10.36 mg·mL-1.The active compounds were extracted,isolated and purified,as an ester of fatty acid.Its molecular weight was identified as 300.1415.

关 键 词：番茄 植物提取物 萝卜蚜 防治效果 

分 类 号：S482.1[农业科学—农药学]