四磺基铁酞菁做为过氧化物模拟酶在过氧化氢及葡萄糖测定中的应用  

Application of Iron-Tetrasulfonatophthalocyanine as a Mimetic Peroxidase to the Determination of Hydrogen Peroxide and Glucose

作　　者：陈秋影[1] 李东辉[1] 郑洪[1] 杨黄浩[1] 许金钩[1] 

机构地区：[1]厦门大学化学系,厦门361005

出　　处：《分析化学》1999年第9期997-999,共3页Chinese Journal of Analytical Chemistry

摘　　要：以四磺基铁酞菁(FeTSPc)作为过氧化物模拟酶催化H_2O_2与L-酪氨酸的荧光反应,建立了测定宽含量范围的H_2O_2及葡萄糖的方法.该法的线性范围为0.0～8.0×10^(-5)mol/LH_2O_2及0.0～2.O×10^(-4)mol/L葡萄糖,检测限分别为5.0×10^(-8)mol/L及6.0×10^(-8)mol/L将该反应与氧化酶催化反应偶联,测定人血清中葡萄糖的含量,取得了满意的结果.Iron(Ⅲ)-tetrasulfonatophthalocyanine (FeTSPc) has been synthesized and used as a mimetic peroxidase to the detennination of hydrogen peroxide and glucose based on its catalytic effect on the oxidation reaction of L-tynosine by hydrogen peroxide. Under optimum conditions, the calibration graphsfor the determination of hydrogen peroxide and glucose were linear over the ranges of 0.0-8.0×10-5mol/L H2O2 and 0.0-2.0×10-4 mol/L glucose, with detection limit of 5.0×10-8 mol/L H2O2 and6.0×10-8 mol/L glucose, respectively. Satisfactory results were obtained when the proposed methodcoupled with the catalysis of glucose oxidase was adopted in the determination of glucose in human sera.

关 键 词：荧光反应 四磺基铁酞菁 过氧化氢 葡萄糖 测定 

分 类 号：O613.3[理学—无机化学] O629.112[理学—化学]