一种适于多肽复合物分析的血红素测定方法  

Determination Method of Heme for Polypeptide Complex Analysis

作　　者：苏冰[1] 王友联[1] 张枢[1] 王宇[1] 龙伟浩[1] 李婷[1] 张瞳[2] 麦荫乔 

机构地区：[1]白求恩医科大学制药厂,吉林长春130012 [2]吉林省康复医疗中心检验科,吉林长春130021 [3]吉深生物技术有限责任公司,吉林长春130021

出　　处：《食品与药品》2011年第3期117-119,共3页Food and Drug

摘　　要：目的建立测定多肽复合物血红素含量的方法。方法将血红素转变为氯化血红素,用紫外分光光度法测定,检测波长399 nm。结果供试品取样量在2～10μg/mL范围内呈良好的线性关系(r=0.999 9),平均回收率(n=6)99.40%,RSD=4.00%。结论该方法简便、灵敏、准确,可作为控制血红素多肽产品质量的方法。Objective To establish a determination method of heme for polypeptide complex analysis.Methods After the heme was conversed to chlorhaematin,the determination of chlorhaematin was carried out by the UV spectrophotometric method and the detection wavelength was 399 nm.Results The linear correlation was good in the sample concentration range of 2～10 μg/mL（r=0.999 9） and the average recovery was 99.40 % with RSD of 4.00 %（n = 6）.Conclusion This method is convenient,sensitive and accurate for the quality control of heme polypeptide products.

关 键 词：多肽复合物 血红素 紫外分光光度法 

分 类 号：R973.6[医药卫生—药品]