3-甲氧基-甲亚胺H光度法测定植物中硼的研究及应用  

STUDY ON THE ABSORPTIOPHOMETRIC DETERMINATION OF BORON IN PLANT WITH l-(2-HYDROXY-3-METHOXYBENZYLIDENE-AMIN() )-8-HYDRO-XYNAPHALENE-3-6-DISULFONIC ACID AND ITS APPLICATION

作　　者：李在均[1] 朱振中[1] 刘玉麒 潘教麦[2] 

机构地区：[1]无锡轻工大学化工系,无锡214036 [2]华东师范大学化学系

出　　处：《理化检验（化学分册）》1999年第9期398-399,401,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：研究了3-甲氧基-甲亚胺H与硼的显色反应。在乙酸-乙酸铵缓冲溶液中。硼与3-甲氧基-甲亚胺H形成1:2的黄色配合物,最大吸收在420nm波长处,摩尔吸尤系数为7.19×10~3,Sandell灵敏度为0.0015/μg·cm^(-2)。当硼含量在0～35μg/25ml范围内符合比耳定律,应用于植物中硼的测定获得满意的结果。l-(2-hydroxy-3-methoxybenzylidene-amino )-8-hydroxynaphalene-3-6-disulfonic acid is a sensitive new chromogenic reagent for boron. In this work, its reaction with B was studied. In an NH_4OAc-HOAc buffer medium of pH 5. 7. B( Ⅲ ) reacts with l-(2-hydroxy-3-methoxybenzylidene-amino)-8-hydroxynaphalene-3-6-disulfonic acid to form a 1 ·2 yellow complex having a maximum absorption at 420nm. Its molar absorptivity is 7. 19X 10~3L ·mol^(-1) ·cm^(-1). Sandell's sensitivity is 0. 0015μg ·cm^(-2). Beer's law is obeyed for 0~35μg B( Ⅲ ) in 25ml. This method has been applied to the determination of B in plants. The results are satisfactory.

关 键 词：硼的测定 植物 光度法 3-甲氧基-甲亚胺H 

分 类 号：Q946.911[生物学—植物学]