肌浆网钙转运ATP酶和受磷蛋白在大鼠心肌梗死后的表达变化  

Changes of SERCA and PLB after acute myocardial infarction in rats

作　　者：张春丽[1] 施冰[2] 曹晶晶[2] 毕磊[2] 

机构地区：[1]解放军总医院第一附属医院骨科实验室,北京100048 [2]北京军区总医院干部病房一科

出　　处：《中国临床保健杂志》2011年第1期57-59,共3页Chinese Journal of Clinical Healthcare

基　　金：国家自然科学基金项目资助(30900829)

摘　　要：目的建立大鼠急性心肌梗死模型,观察心肌梗死后心室重塑过程中肌浆网钙转运ATP酶(Ca-2+ATPase,SERCA)和受磷蛋白(PLB)的表达变化。方法雄性SD大鼠,应用前降支动脉结扎法建立大鼠心肌梗死模型,术后4周观察血流动力学参数,SERCA mRNA和PLB mRNA的表达。结果大鼠心肌梗死后4周,心肌梗死组左心室舒张末压(LVEDP)显著大于假手术组,左心室内压最大上升和下降速率(±dp/dtmax)显著低于假手术组。心肌梗死组心肌组织中SERCA mRNA表达下调,PLB mRNA表达上调。结论大鼠心肌梗死4周后心室收缩和舒张功能已降低,进入了心力衰竭阶段,SERCA mRNA和PLB mRNA的表达变化与心力衰竭的发生发展相关,可能是心肌梗死后心肌收缩和舒张功能失调的重要机制。Objective To observe the changes of sarcoplamic reticulum Ca2＋-ATPase（SERCA）and phospholamban（PLB）during heart failure after acute myocardial infarction in rats.Methods Rats were randomly assigned to sham-operation group and AMI group.4 weeks later,in vivo hemodynamic,SERCA mRNA and PLB mRNA expression of myocardium were measured.Results In comparison to sham-operation group,LVEDP were increased,while maximal rate of rise and fall of left ventricular（±dp/dt max）were decreased in AMI group.The expression of SERCA mRNA were down-regulated（P〈0.05）.The expression of PLB mRNA were up-regulated.（P〈0.05）.Conclusion The changes of SERCA and PLB may be important factors of contractile and dilation dysfunction after AMI.

关 键 词：心肌梗死 心力衰竭 充血性 肌浆网钙转运ATP酶类 蛋白质类 大鼠 Sprague-Dawley 

分 类 号：R542.22[医药卫生—心血管疾病]