青霉素、链霉素体外对人脐带组织源间充质干细胞胞外分泌物基因表达的影响  

Influence of Penicillin and Streptomycin on Gene Expression of Extracellular Secretion from Human Umbilical Cord Tissue Derived Mesenchymal Stem Cells In Vitro

作　　者：李艳萍[1] 时庆[2] 邢晓[2] 汪大琨[2] 庄泳[2] 李栋[2] 

机构地区：[1]山东大学附属省立医院妇产科,山东济南250005 [2]山东大学齐鲁医院低温医学研究室,山东济南250012

出　　处：《中国实验血液学杂志》2011年第1期163-168,共6页Journal of Experimental Hematology

基　　金：山东省自然科学基金资助项目(2006BS03038);国家自然科学基金项目(30700163)资助

摘　　要：本研究探讨青霉素和链霉素对人脐带组织源间充质干细胞增殖、凋亡和胞外分泌物基因表达的影响。首先分离培养人脐带间充质干细胞,然后检测其免疫表型以及向成骨细胞和脂肪细胞分化的能力,并用MTT法检测细胞增殖,定量RT-PCR法检测胞外分泌物(ECS)和凋亡相关基因(bcl-2,bax)的cDNA表达。结果表明:培养得到的贴壁细胞表型符合间充质干细胞特征,低浓度的青霉素和链霉素可以有效促进脐带间充质干细胞的增殖,其最佳作用浓度为100 U/ml;同时青霉素和链霉素能降低其细胞外分泌物(extracellular secretion,ECS)成分的表达,抗生素浓度越高,ECS表达降低越多;而且低浓度的青链霉素还能提高bcl-2/bax的cDNA表达比值。结论:在脐带MSC的体外培养中,低浓度的青霉素、链霉素可增加细胞增殖,降低凋亡比率,但大剂量使用会降低脐带MSC胞外分泌物的基因表达。The study was aimed to investigate the influence of penicillin and streptomycin on proliferation,apoptosis and extracellular secretion（ECS） produced from human umbilical cord derived mesenchymal stem cells（MSC）.MSC were isolated from umbilical cord tissue,then the immunotyping,multipotent differentiation and proliferation of these cells were assayed by cytometry,cytochemistry and MTT respectively.The expressions of ECS and apoptosis-related genes（bcl-2,bax） were detected by quantitative RT-PCR.The results showed that the phenotype of these cells matched with the characteristics of MSC.Penicillin and streptomycin of low concentrations promoted MSC proliferation,with the most effective concentration of 100 U/ml.Expressions of ECS cultured in addition of penicillin and streptomycin were down-regulated.Furthermore,apoptosis-related factor（bcl-2/bax） expression levels in low concentrations penicillin and streptomycin groups were higher than that in the control group.It is concluded that low concentrations penicillin and streptomycin can promote the proliferation and reduce the apoptotic rate,but high dose can inhibit the ECS component expression of MSC.

关 键 词：间充质干细胞 脐带间充质干细胞 青霉素 链霉素 胞外基质 

分 类 号：R978.11[医药卫生—药品] R978.12[医药卫生—药学]