低氧预适应对自体原位肝移植大鼠肠道TLR4表达的影响  

Impact of hypoxia preconditioning on expression of intestinal TLR4 protein in orthotopic liver autotransplantation in rats

作　　者：伍学艳[1] 张培建[1] 庄卓男[1] 孟中良[1] 张杰[1] 苏辉[1] 

机构地区：[1]扬州大学第二临床医学院普通外科研究室,江苏扬州225000

出　　处：《中国现代普通外科进展》2011年第1期9-12,共4页Chinese Journal of Current Advances in General Surgery

基　　金：江苏省卫生厅面上科研课题(H200770);江苏省扬州市科技攻关项目(YZ2008042)

摘　　要：目的:探讨低氧预适应(HP)对大鼠自体肝移植后肠道TLR4蛋白表达的影响。方法:将SD大鼠随机分为正常对照组(NC)、自体肝移植(AT)组和低氧预适应+AT(HP)组3组,每组24只,采用改良的自体肝移植模型。HP组术前用8%氮氧混合气体处理90 min,分别于术后1、6、12 h处死大鼠取小肠标本,抽血检测血清ALT及AST水平,采用免疫组化法测定各组小肠组织TLR4蛋白表达情况,及应用逆转录-聚合酶反应(RT-PCR)检测小肠组织TNF-αmRNA的含量。结果:HP组血清ALT、AST水平,小肠组织TLR4蛋白表达及TNF-αmRNA的含量均显著低于AT组,但均高于NC组,差异均有统计学意义(P<0.05)。结论:HP通过下调肝移植大鼠小肠组织TLR4蛋白的表达,进一步抑制TLR4信号转导通路,减少TNF-α等细胞因子的合成与释放,从而减轻对移植肝功能的损伤。Objective： To investigate the impact of hypoxia preconditioning on the expression of intestinal TLR4 protein after orthotopic liver autotransplantation in rats intestinal.Methods： A modified orthopotic liver autotransplantation model was used to investigate the ischemia reperfusion injury in liver transplantation.Sprague Dawley rats were randomly divided into the following three groups： normal control（NC）group,autotransplantation（AT）group,and hypoxia preconditioning（HP）group,there were 24 rats in each group.HP Group was given an 8% oxygen_mixed gas for 90 minutes before the operation.At 1,6 and 12 hours after operation,ras were killed and following tests were conducted： 1）.blood was drawn to conduct a chemical examination,2）expression of TLR4 protein in intestinal was observed with immunohistochemical stains,3）use RT-PCR to observe the expression of TNF-α mRNA.Results： Serum levels of ALT,AST,and expression of TLR4 protein and TNF-α mRNA in intestinal of HP group were significantly lower than AT group（P0.05） at 1,6 and 12 hours after the orthopotic liver autotransplantation.Conclusion： HP can restrain TLR4 signaling pathway by down regulating the expression of TLR4 protein in orthotopic liver autotransplantation in rats,thus inducing the release of multi-kinds of cytokines like TNF-α and inflammatory mediators,and finally mitigating the damage of transplanted liver.

关 键 词：肝移植 低氧预适应 TLR4 缺血再灌注损伤 

分 类 号：R657.3[医药卫生—外科学]