汉防己甲素体外对正常结膜与翼状胬肉成纤维细胞抑制作用的比较  

Comparison on inhibitory effects of tetrandrine hormone in vitro on normal conjunctiva and pterygium fibroblasts

作　　者：安美霞[1] 吴开力[2] 林少春[2] 潘竹娟[2] 

机构地区：[1]南方医科大学第三附属医院眼科,中国广东省广州市510630 [2]中山大学中山眼科中心国家重点实验室,中国广东省广州市510060

出　　处：《国际眼科杂志》2011年第3期397-399,共3页International Eye Science

基　　金：中国国家自然科学基金资助项目(No.30371513);中国广东省广州市科技计划基金资助项目(No.2007J1-C0101)~~

摘　　要：目的:观察汉防己甲素(tetrandrine,Tet)对正常结膜和翼状胬肉成纤维细胞抑制作用的异同。方法:在相同的培养条件下,传第3代的正常结膜与翼状胬肉成纤维细胞皆加入不同浓度的Tet,分别于加药后1和3d行MTT检测细胞的存活率。结果:用药后1d,4×10-5和2×10-5mol/LTet对正常结膜与翼状胬肉成纤维细胞存活率无显著性差异(P>0.05),其余各浓度的Tet对翼状胬肉成纤维细胞的抑制作用大于正常结膜成纤维细胞,正常结膜成纤维细胞的存活率大于翼状胬肉成纤维细胞(P<0.01)。用药后3d,4×10-5mol/LTet组翼状胬肉成纤维细胞的存活率大于正常结膜成纤维细胞(P<0.01);其余各浓度的Tet对两种成纤维细胞存活率无显著性差异(P>0.05)。结论:用药后3d,2×10-5mol/L及半数抑制量(10-5mol/L)以下浓度的Tet对翼状胬肉成纤维细胞起抑制作用,而对正常结膜成纤维细胞的影响较小。AIM ：To compare the inhibitory effect of tetrandrine（Tet） on fibroblast strains respectively from normal conjunctival and pterygial specimens and provide a new thought in evaluating effect of a drug. METHODS ： Different concentrations of Tet solution were added to the third passage normal human conjunctival flbroblasts （HCF） and human pterygium fibroblasts（HPF）. After 1,3 days in culture, the number of cells was measured by 3-（4,5-dimethyl-2thiazol ）-2,5- diphenyl-2H-tetrazolium bromid （MTT）. RESULTS：At 1 day, there was no statistical difference in the number of HPF and HCF in 4× 10 -5 mol/L and 2 × 10 -5 mol/L Tet tested groups（P 〉 0.05）. The number of HCF was larger than that of HPF in other Tet tested groups （ P〈 0.01）. At 3 day,the number of HPF was larger than that of HCFin 4×10 -5 mol/LTet tested group （P〈0.01）. There was no statistical difference in the number of HPF and HCF in other Tet tested groups（ P〉 0.05）. CONCLUSION In view of the increased proliferation of HPF than that of HCF, 2 × 10 -5mol/L Tet and other lowerdose Tet has damage to the normal HCF when they exhibit significant antiproliferative effect on cultured HPF at 3 day.

关 键 词：成纤维细胞 结膜 翼状胬肉 汉防己甲素 

分 类 号：R285[医药卫生—中药学]