A-FABP基因多态性与肉鸡生长和体组成性状的关联  

Association between chicken A-FABP gene polymorphisms and growth and body composition traits

作　　者：王启贵[1] 关天竹[1] 王守志[1] 张慧[1] 王志鹏[1] 李辉[1] 

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030

出　　处：《遗传》2011年第2期153-162,共10页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30771542);"十一五"国家科技支撑计划重点项目(编号:2008BADB2B01);黑龙江省普通高等学校青年学术骨干支持计划项目(编号:1152G005)资助

摘　　要：为探讨A-FABP基因多态性对肉鸡生长和体组成性状的影响,文章选用肉鸡高、低脂双向选择品系第十世代肉仔鸡为实验材料,采用测序、PCR-RFLP、PCR-LP、DHPLC方法进行基因多态性检测和个体基因型分析,通过对8个SNPs进行连锁不平衡分析并选择5个标签SNPs构建单倍型,进而利用单位点和单倍型分别与鸡生长和体组成性状进行关联分析。结果表明,7个SNPs(除SNP 5以外)及单倍型同时对鸡肌胃重、肌胃率有显著影响(P<0.05),而对生长和其他体组成性状无显著影响(P>0.05)。鉴于不同物种A-FABP基因现有的功能研究均没有发现其对消化系统有重要作用,因此该基因是否为影响肉鸡肌胃重和肌胃率的主效基因还有待于进一步研究。This experiment was designed to study the effects of polymorphism of A-FABP gene on growth and body composition traits in chicken. The 10^th generation broiler population, derived from the Northeast Agricultural University broiler lines divergently selected for abdominal fat content （NEAUHLF） was used. Polymorphism among individuals was detected by DNA sequencing, PCR-RFLP, PCR-LP, and DHPLC. Linkage disequilibrium analysis for eight SNPs was per- formed, and five htSNPs were selected to construct haplotypes. The association analysis between the individual SNPs and haplotypes and growth and body composition traits were investigated, respectively. The results showed that there were con- sistently significant effects on muscle stomach weight （MSW） and percentage of muscle stomach （MSW/BW） （P〈0.05） in the seven SNPs （except for SNP 5） and haplotypes, but no significant effect on any other trait （P〉0.05）. Due to no evidence on effects of A-FABP for digestion system in other species, future experiments need to be developed to confirm whether A-FABP could be a major gene of MSW and MSW/BW traits in broiler chicken.

关 键 词：A—FABP基因 鸡 单倍型 生长 体组成性状 

分 类 号：S831[农业科学—畜牧学]