猪TLR4基因外显子1新等位基因的分离及遗传变异分析  

Isolation of new alleles of the swine TLR4 gene and analysis of its genetic variation

作　　者：潘章源[1] 叶兰[1] 朱璟[1] 杜子栋[2] 黄小国 朱国强[2,3,4] 包文斌[1,3,4] 吴圣龙[1,3,4] 

机构地区：[1]扬州大学动物科学与技术学院,江苏省动物遗传繁育与分子设计重点实验室,扬州225009 [2]扬州大学兽医学院,扬州225009 [3]江苏省现代种猪分子选育工程技术研究中心,常州213149 [4]江苏(扬州)规模猪场高效健康养殖公共技术服务中心,扬州225009

出　　处：《遗传》2011年第2期163-167,共5页Hereditas（Beijing)

基　　金：转基因生物新品种培育科技重大专项(编号:2009ZX08006-004B);江苏省科技支撑计划(农业)(编号:BE2008364,BE2009330-2,BE2010371,BE2010450);江苏省高校自然科学基础研究项目(编号:08KJB230004)资助

摘　　要：文章采用PCR-SSCP方法对亚洲野猪、3个引进的商业化品种和10个中国地方猪品种共893个个体TLR4基因外显子1的遗传变异进行了检测,旨在系统分析国内外猪种TLR4基因的多态性,为探讨该基因在免疫和防御系统中发挥的作用提供依据。结果,在猪TLR4基因外显子1中分离到新的等位基因,共检测到3个等位基因,6种基因型。其中杜洛克检测到AA、BB、CC、AB、AC、BC基因型,有杜洛克血统的苏太猪中检测到BB、CC、BC基因型,长白猪、约克夏中检测到CC、BC基因型,野猪及所有10个中国地方猪品种TLR4基因外显子1高度保守,只检测到CC基因型,中国地方猪品种和引进品种TLR4基因外显子1多态性存在极显著的差异。3种基因型中CC型与GenBank中的序列一致,BB和AA基因型分别存在G93C同义突变位点和G194A无义突变位点,这2个变异位点与抗逆性和一般抗病力的关系值得进一步深入研究。Using the PCR-SSCP method, the genetic variation in exon 1 of the TLR4 gene was detected among 893 animals, including Asian wild boars, 3 imported commercial and 10 Chinese indigenous swine breeds. This was conducted to analyze the polymorphisms of exon 1 of TLR4 gene in native and foreign pig breeds and aimed at providing a theoretical foundation for further research on the role that TLR4 gene played in immune and defense system. New alleles were isolated for exon 1 of the swine TLR4 gene for the first time, There were 6 genotypes and 3 alleles, in which the Duroc appearedAA, BB, CC, AB, AC and BC genotypes; Sutai pig, which has Duroc pig origin, were detected to be BB, CC, and BC genotypes; Yorkshire and Landrace were detected to be CC and BC genotypes. Wild boar and all 10 Chinese native pig breeds appeared highly conserved in exon 1 of TLR4 gene, with only CC genotype. Among the 3 homozygous genotypes, the CC genotype matches the sequence in GenBank, while a G93C synonymous mutation and a G194A nonsense mutation were found in the BB and AA genotypes, respectively. The correlation between these two mututation points of TLR4 gene with resistance to stress and disease is worthy of further study.

关 键 词：TLR4基因 多态性 野猪 地方猪种 引进猪种 

分 类 号：S828[农业科学—畜牧学]