悬钩子皮下盘菌种内遗传多样性的ITS序列分析  

ITS sequence analysis of intraspecific genetic diversity of Hypoderma rubi

作　　者：刘芳[1] 林英任[1] 王士娟[1] 高小明[2] 陈莉[1] 贾国军[2] 

机构地区：[1]安徽农业大学林学与园林学院 [2]安徽农业大学生命科学学院,安徽合肥230036

出　　处：《福建农林大学学报（自然科学版）》2011年第1期54-57,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金(30870014;30770006);教育部高校博士点专项科研基金(20070364002);安徽省自然科学基金(070411005)

摘　　要：通过组织分离法对采自安徽各地的悬钩子皮下盘菌(Hypoderma rubi)鲜材料进行分离培养,共获得12个纯菌株,对各菌株进行DNA提取、rDNA-ITS区PCR扩增和序列测定.序列比较分析表明,悬钩子皮下盘菌ITS序列长度为443-456bp,G+C含量为48.7%-51.5%;不同菌株间的ITS区序列变异较大,该菌种内遗传多样性丰富.同时利用MEGA 4.1软件中的邻接法(neighbour-joining method,NJ)构建系统发育树表明,寄主种类对悬钩子皮下盘菌种内遗传多样性会产生较大影响,木本植物和草本植物上的悬钩子皮下盘菌菌株可能是亲缘关系较远的2个类群;该菌种内遗传差异与其寄生部位和采集地无明显相关性.Fresh materials of Hypoderma rubi collected from Anhui Province were isolated by tissue isolation technique,and 12 pure strains were obtained.DNAs of all the strains were extracted from the dry mycelia,rDNA-ITS regions were amplified by PCR and then sequenced.The ITS sequence analysis results showed that the length range of ITS sequences of H.rubi was from 443 to 456 bp,and the percentage of G＋C contents of the ITS sequences varied from 48.7% to 51.5%.There were obvious variations in the ITS sequences of different strains and showed abundant genetic diversity among them.A phylogenetic tree was constructed by neighbour-joining method using MEGA 4.1.The results indicated that the intraspecific genetic diversity of H.rubi was related to hosts obviously,and strains from woody plants and herbaceous plants probably were two distant relationship groups.In addition,the intraspecific genetic differences of H.rubi were independent of parasitic sites and sample collection area.

关 键 词：皮下盘菌属 模式种 rDNA-ITS序列 遗传多样性 寄主 

分 类 号：Q949.32[生物学—植物学]