一株水产地衣芽孢杆菌的鉴定及培养基优化  

Identification of an aquatic Bacillus licheniformis CJ001 and optimization of the culture media

作　　者：袁科平[1,2,3] 邹世平[1] 李谷[1] 何力[1] 周群兰[2] 

机构地区：[1]农业部淡水生物多样性保护与利用重点开放实验室/中国水产科学研究院长江水产研究所,湖北荆州434000 [2]中国水产科学研究院淡水渔业研究中心,江苏无锡214081 [3]热带亚热带鱼类选育与养殖重点开放实验室/中国水产科学研究院珠江水产研究所,广东广州510000

出　　处：《福建农林大学学报（自然科学版）》2011年第1期69-73,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：中国水产科学研究院基本科研业务费资助项目(2007-008);热带亚热带鱼类选育与养殖重点开放实验室开放课题

摘　　要：从水产养殖环境中分离、筛选得到一株菌CJ001,经菌落形态特征、生理生化特征及16S rDNA测序,鉴定其为地衣芽孢杆菌.地衣芽孢杆菌可有效降低养殖水体中的化学需氧量(COD),48 h内COD的降解率达86.8%,水温30℃时的降解率最高,达到85.7%.为优化培养基组成,选出由单因素试验确定的营养物质(红糖、玉米粉、豆粕、尿素)和NaH2PO4,进行5因素4水平的正交试验.结果表明,CJ001菌株的最佳培养基配方为:红糖3%、玉米粉1%、豆粕3%、尿素0.25%、NaH2PO40.15%,其活菌数可达11.15×109cfu.mL-1.Strain CJ001 was isolated and selected from sediment samples of breed aquatics pond in Wuhan.Based on its major morphologic,physiology-biochemistry characteristics and 16S rDNA sequence analysis,strain CJ001 was identified as Bacillus licheniformis.The strain could degrade the chemical oxygen demand（COD） in aquacultural water effectively,especially at the time of 48 h（with 86.8% degradation rate） or under the temperature of 30 ℃（with 85.7% degradation rate）.According to the results of single-factor orthogonal test for culture optimization,the orthogonal experiment with 5 factors（brown sugar,corn powder,bean,carbamide and NaH2PO4） and 4 levels was then carried out.The optimal medium was as follows： brown sugar 3%,corn powder 1%,bean 3%,carbamide 0.25% and NaH2PO4 0.15%,respectively.Under the condition,11.15×109 cfu bacteria per milliliter were obtained.

关 键 词：地衣芽孢杆菌 水产养殖 化学需氧量 培养基优化 正交试验 

分 类 号：Q939.124[生物学—微生物学]