变应原和激素刺激后变应性鼻炎大鼠Th17细胞及RORγt表达变化  

Change of Th17 and expression of RORγt in a murine model of allergic rhinitis after a stimulation of allergen and corticosteroid

作　　者：瞿中红[1] 李敏[1] 梁建平[1] 袁育林 林志斌[3] 

机构地区：[1]广西壮族自治区人民医院耳鼻咽喉头颈外科,南宁530021 [2]检验科 [3]广州中山大学附属第一医院耳鼻喉科

出　　处：《中华医学杂志》2011年第6期401-404,共4页National Medical Journal of China

基　　金：广西壮族自治区卫生厅科研课题（Z2007151,Z2008045）

摘　　要：目的了解变应原和激素对变应性鼻炎（AR）大鼠Th17细胞和孤独受体（RORTt）mRNA表达水平的影响。方法以含2％氢氧化铝凝胶的屋尘螨提取液复制AR鼠模型后，将屋尘螨提取液对其中10只进行激发，10只进行经鼻吸入皮质类固醇[布地奈德（BUD）]；10只用生理盐水雾化吸入。将处理后AR鼠模型和另10只对照鼠的脾脏细胞和鼻腔黏膜，用流式细胞术检测Th17细胞阳性率、逆转录聚合酶链反应检测RORγt mRNA表达水平、免疫组化检测鼠鼻腔黏膜RORγt表达。结果AR鼠模型鼻黏膜中RORγt表达与正常对照组比较明显上调（25±5比48±10，P〈0．05）。而经BUD处理后AR鼠模型鼻黏膜中RORγt明显下调（48±10比31±6，P〈0．05）。AR鼠模型脾脏细胞中Th17细胞阳性率、RORγt mRNA的表达均高于对照组（18．4％±1．3％比27．5％±1．6％，0．43±0．04比0．64-t-O．05，均P〈0．01）。变应原激发后AR鼠模型脾脏Th17细胞阳性率和RORγt mRNA表达率分别显著低于处理前（27．5％±1．6％比20．0％±2．1％，0．64±0．05比0．54-4-0．03，均P〈0．01）；而BUD处理后二者变化不大（均P〉0．05）。结论变应原疫苗影响变应性鼻炎机体全身免疫功能，可能的机制包括Th17细胞数下降、RORγ mRNA的表达下调来影响机体系统免疫功能；而局部激素对系统免疫作用不大，主要针对鼻黏膜局部免疫起作用，其机制包括RORγt表达下调。Objective To study the effects of allergen and corticosteroid on T help 17 （Th17） and orphan nuclear receptor gammat （RORγt） in allergic-rhinitis mice. Methods Experimental allergic rhinitis （AR） was induced by the extract of dermatophagoides pteronyssinus （DP） including 2% ovalbumin （OVA） sensitization in 30 male mice with DP allergen group （n = 10 ）, intranasal corticosteroid （budesonide, BUD） group （n = 10） or without treatment （model group, n = 10）. And another 10 were included into the normal control group. The murine levels of Th17 were measured by flow cytometry （FCM）. The expression of RORγt mRNA was measured by reverse transcription-polymerase chain reaction （RT-PCR） while that of RORγt protein in nasal mucosa detected by immunohistochemical staining. Results The expression of RORγt in nasal mucosa of AR model was higher than that in the control group （25 ±5 vs 48± 10, P 〈 0. 01 ）. But its expression declined significantly after the administration of local corticosteroid （48 ± 10 vs 31 ±6, P 〈0. 01 ）. The levels of RORγt mRNA and Th17 in the AR model group were significantly higher than those in the control group （ 18.4% ± 1.3% vs 27.5% ± 1.6%, 0.43 ± 0. 04 vs 0. 64 ± 0. 05, both P 〈0. 01 ）. The levels of ROR3,t mRNA and Th17 in the spleen of AR mice were significantly reduced by allergen dosing （27.5%±1.6% vs 20.0% ±2. 1%, 0.64±0.05 vs 0.54±0. 03, both P〈0.01） but not by corticosteroid （P 〉 0. 05 ）. Conclusions Allergen may affect the systemic immunity to inhibit the RORγt expression and block the Th17 differentiation in AR model. Local steroid only produces a marked effect through a down-regulated RORγt expression in nasal mucosa.

关 键 词：鼻炎 变应性 常年性 变应原 转录因子 免疫疗法 激素 

分 类 号：R392.12[医药卫生—免疫学]