增食欲素受体1在营养性肥胖型大鼠胰腺组织中的表达  

Expression of orexin receptor 1 in rat pancreatic tissue with alimentary obesity

作　　者：田雷[1] 赵玉岩[1] 王露[1] 马冬杰[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,沈阳110001

出　　处：《中华医学杂志》2011年第6期409-411,共3页National Medical Journal of China

基　　金：国家自然科学基金（30872724）;辽宁省自然科学基金（20082116）;辽宁省教育厅资助科研项目（L20107125、L2010711）辽宁省科技攻关层次计划项目（2008225009）

摘　　要：目的探讨高脂膳食诱导的肥胖大鼠胰腺组织中增食欲素受体1（OX1R）蛋白的表达规律，阐明OX1R对营养性肥胖型大鼠的体重及代谢指标的影响和调节机制。方法建立高脂膳食诱导的营养性肥胖型大鼠模型；应用微型血糖仪测定全血葡萄糖、生化酶法测定甘油三酯、化学发光免疫分析法测定血清胰岛素的变化；免疫组化鉴定胰腺组织OX1R蛋白的表达；肥胖大鼠侧脑室灌注增食欲素受体1反义寡核苷酸（OX1R—PS—ODNs），检测其对肥胖大鼠胰腺组织中OX1R蛋白产生的影响。结果大鼠饲养8周后，高脂组大鼠体重、血糖、胰岛素、甘油三酯、总胆固醇高于正常组（P〈0．05），大鼠出现营养性肥胖。OX1R蛋白的表达定位于胰腺胰岛β细胞细胞质中，肥胖组OX1R蛋白的表达水平较正常组减低约16％（P〈0．05）。肥胖大鼠侧脑室灌注反义OX1R-PS-ODNs72h后，胰腺组织表达的OX1R蛋白由82-4-6降至65-4-4，下降约21％（P〈0．01）。结论胰岛β细胞中有OX1R蛋白的表达。下丘脑增食欲素系统可以干预肥胖型大鼠胰腺组织中OX1R蛋白的表达，并参与肥胖的发生和血糖、血清胰岛素的代谢调节。Objective To identify the presence of orexin receptor 1 （OXR1） in obese rat pancreas and clarify its effect and mechanism on body weight and metabolic in.dex in obese rats. Methods The obese rat model was established a high-fat diet. And the serum levels of glucose, triglyeride and insulin were measured by Mini-Blood Glucose meter, biochemical enzymatic method and chemiluminescent immunoassay. The pancreatic expression of OX1R protein was bidentified by immunohistochemistry. After an intracerebroventrieular infusion of orexin receptor 1 antisense oligodeoxynucleotide （OX1R-PS-ODNs） , the effect on OX1R protein was detected in rat pancreatic tissue with obesity. Results After feeding for 8 weeks, high-fat diet rats appear alimentary obesity body weight, serum glucose, insulin, triglyeride and cholesterol of high-diet rats were higher than those in the control group （ P 〈 0. 05 ）. The expression of OX1 R protein was located in the cytoplasm of pancreas Islet β-cells. The expression of OX1R protein in obese rat pancreas decreased around 16% versus the normal group （P 〈 0. 05 ）. After the obese rats were treated by OX1R-PS-ODNs for 72 hours, the expression of OX1R protein in pancreas declined from 82 ± 6 to 65±4, reduced about 21% compare with control group （ P 〈 0. 01 ）. Conclusion The expression of OX1R protein is found in pancreas Islet β-cells. Hypothalamic orexin system can intervene the expression of OX1R protein in rat pancreatic tissue with alimentary obesity. And it may participate in the occurrence of obesity and the metabolic regulation of serum glucose and insulin.

关 键 词：肥胖 胰腺 胰岛Β细胞 增食欲素受体1 

分 类 号：R589.2[医药卫生—内分泌]