银屑病患者骨髓CD34＋细胞Notch受体及其靶基因表达的研究  

Expressions of Notch receptors and target genes in bone marrow CD34＋ cells from patients with psoriasis

作　　者：侯瑞霞[1] 李俊琴[1] 刘瑞风[1] 李新华[1] 张静[2] 尹国华[1] 张开明[1] 

机构地区：[1]太原市中心医院皮肤科,030009 [2]太原市中心医院内科,030009

出　　处：《中华皮肤科杂志》2011年第3期168-170,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30771940,30972657）

摘　　要：目的探讨银屑病患者骨髓CD34＋细胞表面两种Notch受体的表达及其下游靶基因Hes-1的表达。方法分离12例银屑病患者及10例正常对照骨髓CD34＋细胞，以B-肌动蛋白为内参照，采用Western印迹法半定量检测Notch受体1、2及其下游靶基因Hes-1的蛋白表达水平。结果银屑病患者CD34＋造血细胞Notchl表达水平（0．9488±0．3221）明显高于正常人对照（0．6693±0．1465）；Hes—I蛋白在银屑病患者表达（0．8579±0．3729）亦高于正常人对照（0．5728±0．1787），两者差异均有统计学意义（P〈0．05），而Notch2蛋白的表达在患者组（0．75684-0．3461）和正常人对照组（0．8312±0．2887）之间差异无统计学意义（P〉0．05）。结论Notchl受体及其下游基因Hes-1的高表达可能参与银屑病患者骨髓造血细胞低增殖活性的发生。Objective To study the expressions of Notch receptors （Notchl and 2） and target gene Hes-1 in bone marrow CD34＋ cells from patients with psoriasis. Methods CD34＋ cells were isolated from the bone marrow of 12 patients with psoriasis and 19 normal human controls. Western blot was conducted to detect the expressions of Notchl, Notch2 and Hes-1 proteins in CD34＋ cells, with the house-keeping gene β-actin as an internal reference. Results In contrast with normal controls, a significant increment was observed in the expressions of Notchl and Hes-1 proteins in psoriatic patients （0.9488± 0.3221 vs. 0.6693 ± 0.1465, 0.8579± 0.3729 vs. 0.5728 ± 0.1787, both P 〈 0.05）. No significant difference was observed in the expression of Notch2 between psoriatic patients and normal controls （0.7568 ± 0.3461 vs. 0.8312 ± 0.2887, P 〉 0.05）. Conclusion The overexpression of Notchl and Hes-1 may be associated with the low proliferation activity of psoriatic hematopoietic cells.

关 键 词：银屑病 抗原 CD34 受体 NOTCH 靶基因 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]