布地奈德对哮喘气道重塑大鼠气道平滑肌细胞间质IL-13及IFN-γ表达的影响  

Effect of budesonide on expressions of IL-13 and IFN-γ in airway smooth muscle cells in rats

作　　者：李淼[1] 尚云晓[1] 

机构地区：[1]中国医科大学附属盛京医院小儿呼吸病科,辽宁省沈阳市110004

出　　处：《江苏医药》2011年第4期378-380,共3页Jiangsu Medical Journal

基　　金：辽宁省教育厅科学研究项目(20060953)

摘　　要：目的探讨布地奈德对哮喘气道重塑大鼠原代培养的气道平滑肌细胞(ASMCs)上清液中白细胞介素13(IL-13)及干扰素γ(IFN-γ)含量的影响。方法 60只Wistar大鼠随机分为三组,每组20只。A组吸入卵清蛋白制作哮喘气道重塑模型,B组在A组基础上隔日1次吸入布地奈德气雾剂,C组吸入生理盐水作对照。8周后取材行苏木素-伊红(HE)染色,测量各组大鼠肺内支气管气道壁面积(Wat)/气道基膜周长(Pbm)及平滑肌层面积(Was)/Pbm;原代培养大鼠ASMCs,ELISA法检测各组ASMCs上清液中IL-13及IFN-γ含量。结果 A、B组Wat/Pbm及Was/Pbm均较C组增加,但B组较A组降低(P<0.05)。A组IL-13含量较C组增加,IFN-γ含量较C组降低(P<0.05);B组IL-13含量较A组降低,IFN-γ含量较A组增加(P<0.05)。结论布地奈德通过降低哮喘大鼠ASMCs间质中IL-13及增加IFN-γ的表达来减轻气道重塑。Objective To investigate the effects of budesonide on the levels of interleukin 13（IL-13） and interferon gamma（IFN-γ） in the supernatant of airway smooth muscle cells（ASMCs） in asthmatic rat.Methods Sixty Wistar rats were randomly assigned into three groups with 20 rats each.The rats in group A were sensitized with ovalbumin aerosol for 8 weeks as asthmatic airway remodeling models and those in group B were given additional budesonide aerosol every other day for 8 weeks.The rats in group C were given saline inhalation as the controls.After 8 weeks the rat lungs were taken for HE staining and image analysis was used to measure wall area normalized by basal membrane perimeter（Wat/Pbm） and smooth muscle layers area normalized by basal membrane perimeter（Was/Pbm） in lobar bronchi.The levels of IL-13 and IFN-γ in the supernatant of ASMCs were detected by ELISA.Results Wat/Pbm and Was/Pbm in groups of A and B were higher than those in group C,while those in group B were lower than those in group A（P0.05）.Compared with group C,the level of IL-13 increased,while IFN-γ decreased,significantly in group A（P0.05）.Compared with group A,the level of IL-13 decreased,while IFN-γ increased,significantly in group B（P0.05）.Conclusion Budesonide is able to decrease the level of IL-13 and increase the level of IFN-γ in ASMCs stroma to alleviate airway remodeling.

关 键 词：哮喘 气道重塑 

分 类 号：R562[医药卫生—呼吸系统]