滋阴清热方对系统性红斑狼疮阴虚内热证患者PBMC基因表达谱的影响  

The Influence in Gene Expression Profiling on PBMC of Internal Heat Due to Yin Deficiency Type SLE Patients with Nourishing Yin Clearing Heat Formula

作　　者：范瑞强 程喜平[2] 赖梅生[3] 吴元胜 陈达灿 禤国维 陈明春[4] 朱晓浚[4] 许德清[4] 

机构地区：[1]广州中医药大学第二附属医院皮肤科,广东广州510500 [2]广州医学院第一附属医院皮肤科,广东广州510500 [3]南方医科大学中医学院中医外科教研室,广东广州510500 [4]中山大学附属第二医院皮肤科,广东广州510500

出　　处：《中国皮肤性病学杂志》2011年第3期225-227,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助项目(30472218)

摘　　要：目的观察滋阴清热方对系统性红斑狼疮(SLE)阴虚内热证患者PBMC基因表达谱的影响。方法用滋阴清热含药血清孵化阴虚内热型SLE患者的PBMC,提取总RNA,用基因芯片检测孵化后PBMC基因表达谱。结果研究发现滋阴清热方含药血清上调51个基因表达,下调92个基因表达,它们分属于21个基因功能簇,基因编码蛋白分布于细胞的8个部位。含药血清和地塞米松共同上调2个基因表达和下调12个基因表达;但是含药血清还下调12个基因表达和上调9个基因表达,而地塞米松对这21个基因无类似调控作用;同样,地塞米松下调11个基因表达和上调15个基因表达,而含药血清对这26个基因无类似调控作用。结论滋阴清热方对SLE阴虚内热型基因表达谱影响是广泛和复杂的,涉及基因数目较多,基因编码蛋白在细胞中分布广泛,具有上调和下调功能簇内基因表达的作用。Objective To observed the influence of nourishing yin clearing heat formula in gene expression profiling on PBMC of internal heat due to yin deficiency type SLE patients. Methods Incubating PBMC of internal heat due to yin deficiency type SLE patients with medicated serum of the nourishing yin clearing heat formula, extracting total RNA and using genechip to detect gene expression profiling of PBMC after incubation. Results The research display： the medicated serum of nourishing yin clearing heat formula had up-regulated 51 genes expression, down-regulated 92 genes expression, all of the genes are belong to twenty-one gene function clusters. And the functional protein ceded by this genes are located at 8 parts of cell. the medicated serum and dexamethasone up-regulated 2 genes expression,downregulated 12 genes expression simultaneously. But medicated serum up-regulated 9 genes expression, down-regulated 12 genes expression the dexamethasone had no similar regulation, however dexamethasone up-regulated 15 genes expression, down-regulated 11 genes expression the medicated serum had no similar regulation too. Conclusion The influence of nourishing yin clearing heat formula in gene expression profiling on internal heat due to yin deficiency type SLE patients were wide and complex. A large number of genes expression were regulated. The functional protein coded by this genes distributed widely in cell.

关 键 词：滋阴清热 阴虚内热 系统性红斑狼疮 基因表达谱 基因芯片 含药血清 

分 类 号：R593.241[医药卫生—内科学]