过氯酸铵对大鼠甲状腺功能及其球蛋白和过氧化物酶mRNA表达的影响  

The effects of ammonium perchlorate on thyroid function and mRNA expression of thyroglobulin and thyroperoxidase

作　　者：巫丰宏[1] 周旋[1] 张娆[1] 潘梅竹[1] 彭开良[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系环境与健康教育部重点实验室,武汉430030

出　　处：《中华劳动卫生职业病杂志》2011年第2期83-86,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（30972452）

摘　　要：目的研究过氯酸铵（AP）对大鼠甲状腺功能及甲状腺球蛋白（强）、甲状腺过氧化物酶（TPO）基因mRNA表达的影响。方法将雄性SD大鼠30只随机分为：正常对照组、低碘组（含碘量：50μg／kg）、AP染毒低（130mg／kg）、中（260mg／kg）、高剂量组（520mg／kg）及AP＋高碘组[AP：520mg／kg，高碘饮水（10mg／L）]，每组5只。经口染毒90d后处死，放射免疫法测定血清游离三碘甲状腺原氨酸（FT3）、游离甲状腺素（n）及促甲状腺激素（TSH）的水平；荧光定量PCR法测定甲状腺球蛋白（Tg）、甲状腺过氧化物酶（TPO）基因mRNA表达水平。结果AP中剂量和AP高剂量组FT4水平[（9．540±1．327）fmol／ml，（6．509±1．949）fmol／ml）]明显低于正常对照组[（13．505±1．276）fmol／ml]，差异有统计学意义（P〈0．05，P〈0．01）。AP高剂量组的TSH水平[（1．227±0．295）mlU／L[明显高于正常对照组[（0．545±0．282）mlU／L]，差异有统计学意义（P〈0．05）；各AP剂量组Tg的mRNA表达相对值均明显低于正常对照组，差异有统计学意义（P〈0．01），AP高剂量组TPO基因mRNA表达相对值明显低于正常对照组，差异有统计学意义（P〈0．05）。结论AP可降低大鼠FT3、FT4水平，引起TSH反馈性增高，并明显抑制Tg和TPO基因mRNA的表达；而高碘可以在一定程度上拈抗AP对大鼠甲状腺的毒作用。Objective To investigate the effects of ammonium perchlorate （AP） on thyroid functions and mRNA expression levels of thyroglobulin （Tg） and thyroperoxidase （TPO） genes of rats. Methods Thirty SD male rats were randomly divided into six groups： control group, iodine-deficient group, low dose AP group （130 mg/kg）, moderate dose AP group （260 mg/kg）, high dose AP group （520 mg/kg） and high iodinecombined group. After the rats were exposed orally for 90 days, serum free-thyroxine （FT4）, free- triiodothyronine （FT3） and thyroid stimulating hormone （TSH） were measured using radioimmunoassays. mRNA expression levels of thyroglobulin （Tg） and thyroperoxidase （TPO） genes were detected by real-time quantitative PCR. Results Serum FT4 levels in moderate dose AP group and high dose AP group were [（9.540± 1.327） fmol/ml] and [（6.509±1.949） fmol/ml] respectively, which were significantly lower than that [ （13.505± 1.276 ） fmol/ml] in control group （P〈0.05 or P〈0.01）. Serum TSH level in high dose AP group was [ （1.227±0.295） mIU/L], which was significantly higher than that [（0.545±0.282） mIU/L] in control group （P〈0.05）. The mRNA expression levels of thyroglobulin （Tg）gene in all groups exposed to AP were significantly lower than that in control group （P〈0.01 ）. The mRNA expression level of thyroperoxidase （TPO） gene in high dose AP group was significantly higher than that in control group （P〈0.05）. Conclusion AP can reduce the serum FT3 and FT4 levels of rats, increase the serum TSH level of rats and decrease obviously the mRNA expression levels of Tg and TPO genes. In addition, high iodine can reduce the toxic effects of AP on thyroid gland of rats to some extent.

关 键 词：过氯酸铵 甲状腺 甲状腺激素类 甲状腺球蛋白 碘化物过氧化物酶 

分 类 号：R1[医药卫生—公共卫生与预防医学]