种间转移扩增法筛选长爪沙鼠微卫星位点  

Screening of novel microsatellite DNA markers in Mongolian gerbils by cross-amplification

作　　者：谭元卿[1] 李薇[1] 杜小燕[1] 路静[1] 吴艳花[1] 王超[1] 陈振文[1] 

机构地区：[1]首都医科大学基础医学院实验动物学系,北京100069

出　　处：《中国实验动物学报》2011年第1期1-5,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(No.30570261);国家科技支撑计划课题(No.2009BAI83B02);北京市教委重点项目(No.KZ200910025002)

摘　　要：目的筛选长爪沙鼠新的微卫星位点,为长爪沙鼠遗传分析提供遗传标记物。方法从GenBank中随机选取小鼠微卫星位点引物536对,用这些引物对长爪沙鼠基因组DNA扩增,将阳性目的条带进行序列分析,找出符合微卫星序列特征的短串联重复序列。结果 536对小鼠微卫星引物在长爪沙鼠基因组中扩增出了313个阳性条带,经序列分析,确定130个长爪沙鼠微卫星位点;其中完美型位点占80.77%(105/130),不完美型位点占19.23%(25/130),与小鼠同源性为24.3%(130/536)。将筛选出的微卫星位点在GenBank中注册,注册号从GU562694到GU562823。结论小鼠和沙鼠的微卫星位点具有较高的同源性,用小鼠的微卫星位点引物直接扩增长爪沙鼠基因组DNA可有效地筛选出长爪沙鼠微卫星位点。Objective To screen new microsatellite loci in Mongolian gerbils to develop genetic markers for genetic analysis.Methods 536 mouse loci were randomly selected from GenBank and amplified in Mongolian gerbils genetic DNA.The positive PCR products were sequenced and confirmed as simple sequence repeat（SSR） loci.Result Of these 536 mouse markers,313（58.39%） were discretely amplified from the laboratory gerbils.Of these 313 sequenced markers,130 were confirmed as SSR loci in the gerbil,in which 105 loci（80.77%） were classified as pure,and 25 loci（19.23%） as unpure.The homology orthologous between mouse and gerbil is about 24.3%（130 /536）,and the access number of loci in GenBank is from GU562694 to GU562823.Conclusions There are comparatively high homology between mouse and gerbil,and cross-amplification by mouse microsatellite primers is an efficient way to identify gerbil SSR loci.

关 键 词：小鼠 长爪沙鼠 微卫星 种间转移扩增 

分 类 号：Q95-33[生物学—动物学]