印楝油两性不育灭鼠颗粒制剂对高原鼠兔抗生育作用的研究  

Studies on the anti-fertility effect of neem oil granules for rodents' ambisexual sterility on Ochotona curzoniae

作　　者：石东霞[1] 蒋忠荣 殷中琼[1,3] 龙兴发 李太强 贾仁勇[1,3] 杨帆[1] 徐娇[1] 张玉群[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]甘孜州畜牧科学研究所,四川康定626000 [3]动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国兽医科学》2011年第2期210-215,共6页Chinese Veterinary Science

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848);四川省科技支撑计划项目(2010N20043);四川省甘孜州科技局项目(200914)

摘　　要：为筛选印楝油两性不育灭鼠颗粒制剂的最优主药量,并探究其对高原鼠兔的抗生育作用,将主药浓度为300g/kg和400g/kg的印楝油两性不育灭鼠颗粒制剂分别进行摄食系数的研究,筛选出适口性较好的制剂,进一步研究适口性较好的制剂对高原鼠兔怀孕率和生殖器官的影响,并用免疫组织化学法检测输卵管和子宫的细胞凋亡情况。结果显示,主药浓度为300g/kg、400g/kg的颗粒制剂对高原鼠兔的摄食系数分别为0.97和1.87,二者之间差异显著(P<0.05);主药浓度为400g/kg的印楝油颗粒制剂对高原鼠兔的抗生育率为70%,对睾丸、附睾、子宫、输卵管和卵巢均有明显的病变影响,并且促进子宫细胞凋亡。结果表明,主药浓度为400g/kg的印楝油两性不育灭鼠颗粒制剂适口性良好,对高原鼠兔有良好的抗生育作用,有望用于草原鼠害的防制。In order to choose the optimal dose of neem oil granules and study its anti-fertility action on Ochotona curzoniae,ingestion coefficients were observed at concentration of 300 g/kg or 400 g/kg of neem oil granules for rodents＇ambisexual sterility,respectively,and the apoptotic cells of oviduct and uterine were measured by immunohistochemical method.In result,the ingestion coefficients at concentration of 300 g/kg and 400 g/kg neem oil granules for rodents＇ambisexual sterility were 0.97 and 1.87 respectively,and the difference was significant compared with the normal control（P0.05）.The anti-fertility rate was 70% on O.curzoniae at 400 g/kg neem oil granules for rodents＇ambisexual sterility,and the dosage caused serious damages on testicle,epididymal duct,hystera,oviduct and ovary,and promoted apoptosis in uterine cells.In conclusion,the 400 g/kg of principle agent concentration of neem oil granules for rodents＇ ambisexual sterility had good palatability and significant anti-fertility effect,which has implications for the control of rodents＇ on grassland.

关 键 词：印楝油颗粒制剂 高原鼠兔 抗生育作用 

分 类 号：S859.799.9[农业科学—临床兽医学]