鹅源H5N1亚型禽流感病毒感染雏鸡免疫器官细胞凋亡的研究  

Apoptosis in the immune organs of chickens infected with H5N1 avian influenza virus derived from goose

作　　者：张瑞莉[1] 刘明[2] 张卓[2] 郑世民[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2011年第3期177-180,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金项目(30371066)

摘　　要：为研究鹅源H5N1亚型禽流感病毒(AIV)人工感染雏鸡免疫器官细胞凋亡的动态变化,本研究将50只1日龄SPF雏鸡随机分为两组。试验组雏鸡于7日龄,分别经鼻、眼、口同时感染105TCID50的鹅源H5N1亚型AIV,分别于感染后3d、4d、5d、7d和14d迫杀,采取胸腺、法氏囊和脾脏,应用TUNEL染色法和透射电镜技术观察其细胞凋亡的动态变化情况。结果显示,试验组雏鸡的胸腺和脾脏凋亡细胞数量在感染后3d～7d极显著高于对照组(p<0.01);法氏囊凋亡细胞数量在感染后3d～4d比对照组明显增加(p<0.05或p<0.01)。组织器官超微结构检测可见凋亡细胞核染色质固缩并凝结成块,聚集在核膜周围,呈新月状或环状;细胞质浓缩。表明鹅源H5N1AIV能够诱导感染雏鸡免疫器官发生细胞凋亡。To investigate the apoptosis in the immune organs of chickens infected with H5N1 avian influenza virus（AIV） derived from goose.Fifty one-day-old specific-pathogen-free chickens were divided randomly into two groups,infected group（I group） and control group（C group）.I group chickens were inoculated with 0.1 mL（105 TCID50） of H5N1 AIV on 7 days old by nasal,eye and oral route simultaneously.The thymus,bursa and spleen from two groups of chickens were taken at 3,4,5,7 and 14 day post inoculation（dpi）,respectively.The apoptosis in those organs was assayed by the terminal deoxyuridine transferase nick-end labeling and transmission electron microscope（TEM）.The result showed that apoptotic cell number in thymus and spleen were significantly higher than that of C group（p0.01） from 3 dpi to 7 dpi.Furthermore,apoptotic cell number in bursa was obviously higher than C group（p0.05 or p0.01） from 3 dpi to 4 dpi.TEM showed that a large number of typical apoptosis cells appeared in the thymus,bursa and spleen,with the morphological characteristics of condensation of nuclear chromatin into dense masses,followed by its margin adjacent to nuclear membrane,and concentration of cytoplasm.These results suggested that goose origin H5N1 AIV could cause apoptosis in the immune organs of infected chickens.

关 键 词：禽流感病毒 免疫器官 细胞凋亡 

分 类 号：S852.65[农业科学—基础兽医学]