鸡IL-1β、IL-16基因真核表达质粒对IBV DNA疫苗的免疫增强效应研究  

Enhancement of protective efficacy of IBV DNA vaccine by eukaryotic recombinant plasmids expressing chicken IL-1 beta and IL-16

作　　者：张毅[1] 王红宁[1] 樊汶樵[1] 张安云[1] 曹海鹏[1] 郭自成[1] 丁梦蝶[1] 

机构地区：[1]四川大学生命科学学院动物疫病防控与食品安全四川省重点实验室/生物资源与生态环境教育部重点实验室/"985工程"西南资源环境与灾害防治科技创新平台,四川成都610064

出　　处：《中国预防兽医学报》2011年第3期214-218,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：十一五国家高技术研究发展计划(2006AA10A205);现代农业产业技术体系建设专项资金

摘　　要：为研究鸡IL-1β、IL-16基因真核表达质粒对鸡传染性支气管炎病毒(IBV)DNA疫苗免疫效果的影响,根据GenBank登录的鸡IL-1β和IL-16序列设计两对引物,采用RT-PCR方法从四川山地乌骨鸡外周血淋巴细胞RNA中克隆IL-1β和IL-16基因,与真核表达质粒pcDNA3.1(+)连接构建了pDNAIL-1β和pDNAIL-16。分别将两种重组质粒与IBVDNA联合免疫SPF雏鸡并进行攻毒试验。结果表明,pDNAIL-1β和pDNAIL-16均能促进血清中特异性抗体水平和外周血T淋巴细胞亚群数量的增加(p<0.05),增强IBV DNA疫苗对同型强毒的保护率15%～25%,pDNAIL-1β提升DNA疫苗的抗体水平的时间比pDNAIL-16多7d。试验表明,鸡IL-1β、IL-16基因真核表达质粒对IBV DNA疫苗的免疫具有增强作用。To enhance the immune efficacy of infectious bronchitis virus（IBV） DNA vaccine,eukaryotic recombinant plasmids expressing IL-1β and IL-16 of Sichuan Mountainous Black-bone chicken,pDNAIL-1β and pDNAIL-16,were constructed and utilized to co-inoculate 7-day-old SPF chicken with IBV DNA vaccine as molecular adjuvants.Five weeks post inoculation,chickens were challenged by virulent IBV strain.The co-inoculation of either molecular adjuvants induced a higher level of specific antibody and the ratio of CD3＋,CD4＋ and CD8＋ T lymphocytes in perpheral blood,and provided an higher protection rate（25% or 15%） against the virulent IBV strain than the group inoculated only with DNA vaccine.Moreover,the enhancing effect of pDNAIL-1β was found to last at least 7 day longer than that of pDNAIL-16.These results indicated that pDNAIL1β and pDNAIL16 could be used to improve the efficacy of IBV DNA vaccine through co-administration with the vaccine.

关 键 词：禽传染性支气管炎病毒 鸡IL-1β 鸡IL-16 免疫增强作用 

分 类 号：S852.4[农业科学—基础兽医学]