副猪嗜血杆菌黑龙江株的分离与鉴定  

Isolation and identification of Haemophilus parasuis in Heilongjiang area

作　　者：李雪松[1,2] 陈欣[2] 符芳[2] 王伟[3] 田华彬[2] 郎越坤[2] 徐春红[1] 李曦[2] 李春艳[1] 

机构地区：[1]东北农业大学,黑龙江哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨150001 [3]哈尔滨师范大学,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2011年第3期236-238,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：黑龙江省自然基金重点项目(ZJN0503-02)

摘　　要：为确定黑龙江某猪场发病猪群的病原,本研究从疑似浆膜炎的病料中分离到一株革兰氏阴性细小杆菌,通过对其进行细菌形态学、培养特征、生化特性和16S rRNA基因PCR鉴定,结果表明该分离菌株为副猪嗜血杆菌(HLJ-1分离株)。16S rRNA基因序列分析结果显示:该分离菌株与参考株的基因同源性达99%;药敏试验结果表明HLJ-1分离株对头孢曲松、头孢噻肟等β-内酰胺类抗生素高度敏感;小白鼠致病性试验结果显示,该分离株具有较强致病性。A small polymorphic gram negative bacterium was isolated from affected pig in Heilongjiang area.The bacterium was identified as Haemophilus parasuis（HLJ-1） by bacteria morphology,cultural character,physiological and biochemical characteristics and 16S rRNA gene PCR.Result revealed that 16S rRNA gene of HLJ-1 isolate shared the homology of 99% with reference strains.Drug sensitive tests showed that HLJ-1 was highly sensitive to β-lactam antibiotics,such as Ceftriaxone and Cefotaxime,and less sensitive to polymixin B,but resistant to Phytomycin.Furthermore,pathogenic test in mice indicated that HLJ-1 isolate was a highly virulent.

关 键 词：副猪嗜血杆菌 分离鉴定 16S RRNA基因 

分 类 号：S852.61[农业科学—基础兽医学]