AGEs对心肌微血管内皮细胞血管新生和管腔形成的影响  

Effect of advanced glycation end products on angiogenesis and tube formation of microvascular endothelial cells

作　　者：粟妍晖[1] 李飞[1] 刘楠[1] 王东娟[1] 王海昌[1] 

机构地区：[1]第四军医大学西京医院心血管内科,陕西西安710032

出　　处：《心脏杂志》2011年第1期42-45,共4页Chinese Heart Journal

摘　　要：目的:观察糖基化终产物(advanced glycation end products,AGEs)对心肌微血管内皮细胞(cardiac microvascu-lar endothelial cells,CMECs)增殖能力、迁移能力,以及血管新生和管腔形成的作用和影响。方法:以不同浓度(100、200和400 mg/L)的AGEs作用于CMECs 48 h,分别采用MTT比色法检测细胞的增殖能力;用Transwell法检测细胞的迁移能力;用毛细血管管腔结构形成试验检测AGEs对血管新生的影响。结果:CMECs在以不同浓度(100、200及400 mg/L)的AGEs作用48 h后,MTT比色法检测显示,吸光值(分别为0.1195±0.0049、0.1422±0.0058及0.1783±0.0220)明显高于对照组(0.0955±0.0161,P<0.05,P<0.01);Transwell法检测细胞数[分别为(24.89±6.234)、(32.89±6.990)及(55.56±10.27)个]均高于对照组[(13.89±4.622)个,P<0.05,P<0.01];管样结构的长度[分别为(3.261±0.7016)、(4.737±1.129)及(6.687±1.308)mm/mm2]均高于对照组[(2.089±0.6723)mm/mm2,P<0.05,P<0.01]。结论:AGEs可以促进CMECs血管新生和管腔结构的形成,且与其浓度呈正相关。AIM： To investigate the effects of the advanced glycation end products（AGEs） on the proliferation,migration and capillary tubule-like formation（TLS） in cardiac microvascular endothelial cells（CMECs）.METHODS： CMECs were cultured with AGEs at different concentrations（100 mg/L,200 mg/L,and 400 mg/L） for 48 h.MTT and transwell were used to detect the proliferation and migration and capillary tubule-like formation was examined to detect the angiogenesis.RESULTS： The A values of CMECs at the AGEs concentration of 100 mg/L,200 mg/L and 400 mg/L after 48 h incubation were 0.1195±0.0049,0.1422±0.0058 and 0.1783±0.0220,significantly higher than 0.0955±0.0161（P〈0.05,P〈0.01）.The migrations of CMECs（24.89±6.234,32.89±6.990 and 55.56±10.27） were higher than 13.89±4.622（P〈0.05,P〈0.01）,and the tubule-like formation structures of CMECs were 3.261±0.7016,4.737±1.129 and 6.687±1.308,higher than 2.089±0.6723（P〈0.05,P〈0.01 vs.control group） in a concentration dependent manner.CONCLUSION： AGEs promote the proliferation,migration and tubule-like structure formation in a concentration-dependent manner.

关 键 词：糖基化终产物 心肌微血管内皮细胞 血管新生 管腔形成 

分 类 号：R587.2[医药卫生—内分泌]