HPLC法测定氢化可的松戊酸酯有关物质  

Determination of hydrocortisone valerate related substances by HPLC

作　　者：陈丽萍[1] 殷义平 王元有[1] 张杰[1] 

机构地区：[1]扬州工业职业技术学院,扬州225127 [2]江苏联环药业集团有限公司,扬州225009

出　　处：《分析仪器》2011年第2期27-29,共3页Analytical Instrumentation

摘　　要：用HPLC法测定了氢化可的松戊酸酯有关物质,结果表明,氢化可的松戊酸酯与氢化可的松及氢化可的松-21-戊酸酯分离良好,氢化可的松戊酸酯与氢化可的松及氢化可的松-21-戊酸酯的最低检测限分别为0.9ng,0.2ng,2.0ng。本法专属性强,结果准确、灵敏,操作简便,可作的质量控制方法。Objective： To establish a HPLC method for the determination of hydrocortisone valerate and related substances.Method： A Phenomenex C18 column（250mm×4.6mm,5μm） was used with ACN-water（45 ︰55） as mobile phase.The flow rate was 0.8mL/min,the column temperature was 30℃,and the detection was at 290nm.Results： Hydrocortisone valerate was separated from impurities（hydrocortisone and hydrocortisone-21-valerate） completely.Their detection limits for hydrocortisone valerate,hydrocortisone and hydrocortisone-21-valerate were 0.9ng,0.2ng and 2.0ng,respectively.Conclusion： The method is accurate,sensitive,simple and specific,and is appropriate for the quality control of hydrocortisone valerate.

关 键 词：高效液相色谱法 氢化可的松戊酸酯 氢化可的松 氢化可的松-21-戊酸酯 

分 类 号：R927.2[医药卫生—药学]