活血益智片对血管性痴呆小鼠凋亡蛋白p53及caspase-3表达的影响  

Effect of Huoxueyizhi slice on p53 and caspase-3 protein expression in hippocampus of vascular dementia mice

作　　者：王丽红[1] 文慧[2] 齐越[3] 贾冬[3] 周绮[4] 鄂蕴娟[3] 

机构地区：[1]鞍山市中心医院中医科,辽宁鞍山114001 [2]沈阳医学院奉天医院干诊科,沈阳110024 [3]辽宁中医药大学,沈阳110032 [4]上海中医药大学药物安全性评价中心,上海201203

出　　处：《中国临床药理学杂志》2011年第3期199-201,共3页The Chinese Journal of Clinical Pharmacology

基　　金：沈阳科学技术计划基金资助项目(1091141-9-00)

摘　　要：目的研究活血益智片(抗血管性痴呆中成药)对血管性痴呆小鼠(VaD)认知功能及海马神经元凋亡蛋白的影响。方法用反复缺血再灌注小鼠模型,模拟VaD行为及病理过程,观察3个剂量(2.24,4.48,9.96 g.kg-1)活血益智片组及氢麦角碱组7.8 mg.kg-1连续给药28 d后,对海马区学习记忆成绩、p53及caspase-3蛋白表达的影响。结果活血益智片组与模型组比较,可提高小鼠的学习记忆能力;降低p53及caspase-3蛋白表达。结论活血益智片可通过调节p53及caspase-3蛋白表达而发挥抗凋亡作用。Objective To observe the effect of Huoxueyizhi slice on memory impairment and expression of p53 and caspase-3 in hippocampus of vascular dementia（VaD） mice.Methods Vascular dementia（VaD） mice model was made by two times of transient ischemia and reperfusion of bilateral common carotid arteries plus tail bleeding.The memory test,p53 and caspase-3 protein expression in hippocampus of VaD mice treated with and without Huoxueyizhi slice（2.24,4.48,9.96 g·kg-1） and hydergine（7.8 mg·kg-1）,continuous administration of 28 days,were measured.Results Compared with model group,Huoxueyizhi slice significantly improved memory impairment.Meanwhile,based on the determination in hippocampus through method of the western blot,Huoxueyizhi slice decreased the expression of the proapoptotic protein p53 and caspase-3.Conclusion Huoxueyizhi slice can exert antiapoptotic effect through regulating p53 and caspase-3 protein expression.

关 键 词：血管性痴呆 活血益智片 记忆障碍 海马 凋亡 

分 类 号：R285.5[医药卫生—中药学] R977.9[医药卫生—中医学]