广东省平胸龟遗传多样性的RAPD分析  

Genetic Diversity Analysis of Platysternon megacephalum in Guangdong Province by RAPD Technique

作　　者：于冬梅[1] 彭建军[1] 沈梓粤[2] 王莹[2] 

机构地区：[1]华南濒危动物研究所物种鉴定中心,广州510260 [2]暨南大学生命科学技术学院,广州510632

出　　处：《生物技术通报》2011年第3期125-129,共5页Biotechnology Bulletin

基　　金：广东省自然科学基金项目(9151026001000003);广东省科学院优秀青年科技人才基金项目(200704);广东省野生动物保护和利用公共实验室基金项目(200901)

摘　　要：由于栖息地的破坏和人为的滥杀滥捕,平胸龟野外资源数量急剧下降,现已处于濒危状态。将13条可重复的、扩增图谱清晰的RAPD引物用于广东省内20个平胸龟个体的遗传多样性分析。扩增所得的条带显示,多态性位点含量为60.71%,Nei’s基因多样性指数为0.1196,Shannon’s多样性信息指数为0.1966,表明平胸龟遗传多样性仍较丰富。同时,计算20个个体间遗传距离为0.0507-0.2925,并采用UPGAM方法绘制聚类分析树状图,20个个体聚类较分散,主要聚为1个大群体,表明广东省内的野生平胸龟并没有形成明显的种群的分化。了解平胸龟的遗传现状、种群结构,可为该物种的种质资源保护、野生资源恢复与利用提供理论依据。The big-headed turtle（Platysternon megacephalum）has become increasingly more endangered due to habitat loss and poaching.Here,genetic diversity of 20 individuals sampled in Guangdong was analyzed using 13 arbitrary primers.The proportion of polymorphic loci was 60.71%,with the Nei＇s gene diversity and the Shannon information index was 0.1196 and 0.1966,respectively.The genetic distances among 20 individuals were found to be 0.0507 to 0.2925.The dendrogram,obtained by UPGMA analysis,showed that 20 samples could be one group.In order to make protection measures for conservation or recovery of wild resources,the genetic diversity and population structure should be studied.

关 键 词：平胸龟 遗传多样性 RAPD 

分 类 号：S917.4[农业科学—水产科学]